20.500.12556/RUL-92925 ZMANJŠANJE TEMPERATURNIH RAZLIK V BIOREAKTORJIH AMBR TEMPERATURE DIFFERENCES REDUCTION IN BIOREACTORS AMBR Bioreaktor je posoda v kateri ustvarjamo čim boljše pogoje za celice, enocelične organizme ali encime, da iz preprostih kemikalij ustvarjajo nam koristne produkte. Pri tem procesu dobimo tudi bolj ali manj želene stranske produkte. V podjetju Lek d.d. uporabljajo dva sistema bioreaktorjev Ambr, od katerih ima vsak po štiri sklope dvanajstih bioreaktorjev. Uporaba je pokazala, da je temperatura v posameznih bioreaktorjih sklopa različna, kar povzroča neenakomerno rast celične kulture v bioreaktorjih. Da bi izboljšali fizikalno-biološke pogoje v posameznem sklopu, smo za sistem bioreaktorjev Ambr izdelali grelni sistem, ki zmanjša temperaturne razlike med bioreaktorji sklopa. V tokokrog smo vezali ploščat grelec, napajalnik in stikalo. Na grelec smo namestili senzor temperature ter njegov signal pripeljali na mikrokrmilniško razvojno ploščico. Glede na signal temperaturnega senzorja mikrokrmilniška razvojna ploščica s pulzno širinsko moduliranim signalom ustrezno uravnava delovanje grelca. Grelni sistem smo nadgradili z modulom s pomnilniško kartico, ki beleži delovanje grelca. Testne meritve so pokazale, da je zasnovan grelni sistem uspešna rešitev omenjenega problema. Temperaturne razlike med najhladnejšim in najtoplejšim reaktorjem sklopa zmanjša dva do dva in pol krat, odvisno od nastavljene temperature sklopa. Nadgradnja in dopolnitev sistema bo doprinesla k njegovi robustnosti in varnosti. A bioreactor is a receptacle in which we maintain optimal conditions that enable cells, unicellular organisms or enzymes to produce useful products from simple chemicals. In the process we can also acquire some more or less desired by-products. The firm Lek d.d. utilizes two bioreactor systems Ambr, each with four blocks of twelve bioreactors. During their employment it became clear that the temperature in the bioreactors within one block differs, which in turn resulted in an uneven growth of cells. In order to improve physical and biological conditions within one block, we have constructed a heating system for the bioreactor systems Ambr that diminishes the temperature differences within a block of bioreactors. We joined the heater, a charger and a switch into a circuit. Onto the heater we attached a temperature sensor and directed its signal to a microcontroller control panel. The microcontroller control panel thus adequately regulates the heater with a pulse-width modulation signal according to the signal received from the temperature sensor. We enhanced the heating system by adding a module for a memory card, which records the heater’s performance. Test measurements have shown that the heating system has proved itself as a successful solution to the problem posed. It diminishes the temperature differences between the coldest and warmest of the bioreactors within a block the bioreactors two to two and a half times, depending on the temperature that has been set for the block. Further additions and upgrading of the system will make it more robust and safe. bioreaktor Ambr ploščati grelec celična kultura bioreactor Ambr flat heater cell culture true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2017-07-10 14:55:04 2017-07-10 14:55:10 2022-08-10 01:20:36 0000-00-00 00:00:00 2017 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 38945 Staric_Miha_-_ZMANJSANJE_TEMPERATURNIH_RAZLIK_V_BIOREAKTORJIH_AMBR.pdf Staric_Miha_-_ZMANJSANJE_TEMPERATURNIH_RAZLIK_V_BIOREAKTORJIH_AMBR.pdf 1 6AD4B297086B8F38D649EE56C284B4D9 339a03879b90a012c53809c43094c1104235f96ffe4f5c5fd023841c098a9b67 75c97f89-a1b3-11eb-a523-00155dcfd717 20.500.12556/rul/b1672569-5fc5-4c06-854a-700eca659358 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=101091 Fakulteta za elektrotehniko 0 0 0