20.500.12556/RUL-86355 Izbris gena za egerolizin v bakterijskem sevu Pseudomonas aeruginosa PA01 Deletion of aegerolysin gene in strain of bacteria Pseudomonas aeruginosa PA01 V diplomskem delu predstavim proteine iz družine egerolizinov in se osredotočim na protein RahU bakterije Pseudomonas aeruginosa. Bakterija P. aeruginosa je oportunistični patogen, ki izkorišča šibkost gostiteljevega imunskega sistema in povzroča številne okužbe. Predstavim pomen produkta gena pri obrambi bakterije. Osredotočim se na pripravo delecijske mutante bakterije P. aeruginosa. Deletirala sem gen rahU v genomu bakterije P. aeruginosa, tako da je v mutanti zamenjan z genom za odpornost proti antibiotiku kanamicinu. Zamenjavo genov sem izvedla s homologno rekombinacijo z rekombinaznim sistemom Lambda Red. Namen uporabe omenjenega molekularnega orodja je uspešna pridobitev delecijske mutante, ki nam v nadaljnjih analizah služi za prepoznavo funkcije proučevanega proteina z neznano funkcijo, naprimer proteina RahU. The dissertation discusses the proteins from the aegerolysin family, specifically the RahU protein of the opportunistic pathogen bacteria, Pseudomonas aeruginosa. The biology of P. aeruginosa, which exploits its host’s weak immune system to cause numerous infections, is described. The main focus of the dissertation was the preparation of the deletion mutants. The rahU gene was deleted from the P. aeruginosa genome by replacing it with the kanymicin cassette. The gene replacement was carried out by using the Lambda Red recombinase system. The purpose of this molecular tool was to gain the rahU deletion mutants, which enable us to obtain new insights into the biology of the RahU protein. egerolizini aegerolysins true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2016-10-08 02:41:51 2017-09-19 15:25:14 2025-04-17 04:52:36 0000-00-00 00:00:00 2016 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 11224649 Diplomsko_delo_Melisa_Trsan,_Ljubljana_2016.pdf Diplomsko_delo_Melisa_Trsan,_Ljubljana_2016.pdf 1 3EF3CDBBB1F5493252DAD2A1CC5920E3 0860bf5f908221596284ebbafbedd4f042b435c297d6c9b6d1daad5584dd8130 05a4f1ea-1aac-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=204946 Pedagoška fakulteta 0 0 0