20.500.12556/RUL-85997 Acetilholinesterazna aktivnost v vodnem ekstraktu gobe bukov ostrigar (Pleurotus ostreatus) Acetylcholinesterase activity in water extract of the oyster mushroom (Pleurotus ostreatus) Zanimalo nas je, ali je v vodnem ekstraktu gobe bukovega ostrigarja (Pleurotus ostreatus) mogoče zaznati aktivnost encima acetilholinesteraze (AChE), če kot substrat uporabimo acetiltioholinklorid (AChCl). Z dodatkom različnih koncentracij substrata in različnih inhibitorjev, smo želeli proučiti, v kolikšni meri ti vplivajo na aktivnost encima. Uspeli smo dokazati aktivnost encima AChE v vzorcih bukovega ostrigarja. Aktivnost je lineano naraščala s povečevanjem koncentracije substrata AChCl. Pri koncentraciji 1 mM AChCl inhibitorji eserin, neostigmin bromid in poli APS ne zavirajo aktivnosti encima. Predlagamo nadaljnje študije z drugimi substrati, ki vsebujejo tioholin ter študije inhibicije encima pri višjih koncentracijah substrata acetilholinklorida. We were interested to see, if there is a possibility to detect any activity of the enzyme acetylcholinesterase (AChE) in the water extract of the oyster mushroom (Pleurotus ostreatus), if acetylthiocholine chloride (AChCl) is used as a substrate. We wished to study at what rate the enzyme activity is affected, if we add different concentrates of the substrate and different inhibitors. In samples of the oyster mushroom, activity of the enzyme AChE was successfully proven. The activity linearly grew in correspondance with increasing concentration of the AChCl substrate. Inhibitors eserine, neostigmine bromide, and poly APS at 1 mM concentration of AChCl, do not block the enzyme activity. We suggest further studies with other substrates, containing thiocholine. We also suggest studies of the enzyme inhibition at higher concentrations of substrate acetylthiocholine chloride. bukov ostrigar oyster mushroom true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2016-10-04 02:42:29 2017-09-19 17:27:15 2025-04-17 04:52:34 0000-00-00 00:00:00 2016 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 11204169 Diplomska_naloga_BERGANT_Tjasa.pdf Diplomska_naloga_BERGANT_Tjasa.pdf 1 C17C6790C6A7662EAA334EC25CBFA612 d5644e11a227b669478a36810852bba2dc598d011b0d3ad06fbe28378b36e3f7 e3e8cab3-1aab-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=204884 Pedagoška fakulteta 0 0 0