20.500.12556/RUL-69610 Vpliv onesnaževanja ozračja na genetsko strukturo bukovih populacij v Sloveniji The effect of air pollution on genetic structure of beech populations in Slovenia Raziskava je zajela osemnajst populacij bukve (Fagus sylvatica L.), dvanajst iz relativno čistih in šest iz onesnaženih naravnih gozdnih sestojev. Šest populacij, od tega tri čiste in tri onesnažene, je bilo mladih. Analizirali smo naslednjih dvanajst izoencimskih lokusov: GOT-8, IDH-A, LAP-A, MDH-A, MDH-8, MDH-C, MNR, PX-A, PX-B, PGM, PGl-8 in SKDH. Na lokusih LAP-A in IDH-A smo odkrili značilne razlike med alelnimi frekvencami skupine čistih in skupine onesnaženih populacij. Genetske distance med starševsko populacijo in njenim potomstvom so na onesnaženi ploskvi v vseh primerih večje kot na čisti. Onesnaževanje je najverjetneje povzročilo bistveno spremembo alelnih frekvenc, kar se kaže tudi v dejstvu, da so genetske distance največje, kadar primerjamo onesnažene populacije s čistimi ali kadar primerjamo onesnažene populacije med seboj. Nekatera dejstva nakazujejo selekcijo proti določenim alelom, toda nedvoumnih dokazov selekcije raziskava ni odkrila. Eighteen European beech (Fagus sylvatica L.) populations, twelve of which are relatively undamaged and six severely damaged, were analyzed. Six populations were young; three of them polluted and three unpolluted. The following twelve isozyme loci were used: GOT-8, IDH-A, LAP-A, MDH-A, MDH-B, MDH-C, MNR, PX-A, PX-6, PGM, PGI-B and SKDH. Significant differences of allelic frequencies between the groups of polluted and unpolluted populations on loci LAP-A and IDH-A were determined. The genetic distances between the old population and its progeny are, in ali cases, higher on polluted sites than on unpolluted ones. It is very likely that pollution has caused a substantial change in allelic frequencies, which is also indicated by greater genetic distances between polluted and unpolluted populations as well as between polluted populations only. There are some indications that selection against some alleles is present, but this was not unambiguously confirmed in the study. bukev zrak onesnaževanje izoencimi genetska struktura genetska variabilnost genetska raznolikost genetska diferenciacija European beech air pollution genetic structure isozymes genetic variability genetic diversity genetic differentiaton true false false Slovenski jezik Angleški jezik Znanstveno delo 2015-07-10 21:33:02 2015-07-10 21:33:02 2024-08-10 03:59:06 0000-00-00 00:00:00 1996 0 0 Str. 67-103 Št. 49 1996 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 630*18:630*16+630*17Fagus sylvatica (L.)(497.4)(045)=163.6 0351-3114 170150 Brus_1996.pdf Brus_1996.pdf 1 951499AEC90C490885BF21EC93C066CD f5c02841437cff6e06c4996f7d5828cbdd73086ee73dfd83db1dbcebb3378936 682413cc-a1b3-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=100835 Biotehniška fakulteta 0 0 0