20.500.12556/RUL-69147 DNazna aktivnost DNaze I iz goveje trebušne slinavke in podenote B bakterijskega citotoksina CdtABC diplomsko delo DNase activity of DNase I from bovine pancreas and B subunits of the bacterial cytotoxin CdtABC DNaza je encim, ki ga uvrščamo med endonukleaze. To je najbolj preučeni encim med endonukleazami, ki razgrajuje dvoverižno DNA. Poleg navedene funkcije se DNaza uporablja tudi kot orodje v molekularni biologiji in, tudi pri zdravljenju cistične fibroze. Citotoksini CdtABC so skupina proteinskih toksinov, ki so prisotni pri bakterijah iz družine Proteobacteria. Zaradi DNazne aktivnosti so genotoksični za evkariontske celice V svoji diplomski nalogi sem primerjala aktivnost encima iz skupine endonukleaz, to jegovejo pankreasno endonukleazo DNazo I s podenoto CdtB iz bakterije Aggregatibacter actinomycetemcommitans. Osredotočila sem se na primerjavo dveh encimskih metod,in na pogoje, ki so potrebni za aktivnost DNaze I in podenote B iz citotoksina CdtABC. DNase is an enzyme belonging to the endonuclease group. It is the most examined enzyme among endonucleases that degrades double-stranded DNA. In addition, DNase is used as a tool in molecular biology and for treating cystic fibrosis. Cytotoxins CdtABC are a group of protein toxins that are present in bacteria from the Proteobacteria family. Owing to their DNase activity, they are genotoxic to eukaryotic cells. The thesis compares the activity of an enzyme from the endonuclease group, i.e. bovine pancreatic endonuclease DNase I with the CdtB subunit from the bacteria Aggregatibacter actinomycetemcommitans. We focused on the comparison of two enzymatic methods and the conditions necessary for the activity of DNase I and subunit B from the CdtABC cytotoxin. encimi endonukleaze DNaza I DNaza II citotoksin Cdt encimska aktivnost true false false [D. Durić] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2015-07-10 21:19:10 2015-07-10 21:19:10 2025-04-17 04:52:08 0000-00-00 00:00:00 2014 0 0 30 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.645.4(043.2) 10236233 Denisa_Duric_diplomsko_delo.pdf Denisa_Duric_diplomsko_delo.pdf 1 298A9B9923765FFFE3D985600A30B6B1 0656fef7daad27ab46e6f1521a6a063f65af2aa7a6e768e91373afaf4909819e c494c806-1aa6-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=203848 Pedagoška fakulteta 0 0 0