20.500.12556/RUL-61563 Enumration, isolation, and identification of bifidobacteria from dairy products Štetje, izolacija in identifikacija bifidobakterij v mlečnih izdelkih Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg l-1). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46 °C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification was also carried out by PCR method using genus- and species-specific primers. Testirali smo šest mlečnih izdelkov. Štetje bifidobakterij in izolacijo smo izvedli na TPY agarju, ki smo mu dodali muriprocin (100 mg l-1). Izolate smo identificirali na ravni rodu z detekcijo fruktoza-6-fosfat fosfoketolaze (F6PPK) in z metodo FISH. Bifidobakterije smo opisali s hitrima testoma API 50CHL in API ID 32. Preverili smo tudi rast pri 46 °C. Nato smo vse isolate identificirali na ravni vrst z računalniškim programom Bacter. Dodatno smo izvedli identifikacijo z metodo PCR ob uporabi za posamezen rod, oziroma vrsto specifičnih začetnih oligonukleotidov. milk products dairy products microbiology bifidobacteria enumeration identification isolation mlečni izdelki mikrobiologija bifidobakterije štetje identifikacija izolacija true false false Biotehniška fakulteta Angleški jezik Slovenski jezik Delo ni kategorizirano 2015-07-10 19:04:07 2015-07-10 19:04:07 2022-07-05 03:43:16 0000-00-00 00:00:00 2004 0 0 Str. 31-36 št. 1 Letn. 84 2004 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 637.1 1581-9175 1677448 213840640 http://www.dlib.si/details/URN:NBN:SI:doc-YY3L09RD 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=61574 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0