20.500.12556/RUL-60776 Toxic and genotoxic potential evaluation of soil samples by bioassays Vrednotenje strupenosti in genotoksičnosti vzorcev zemlje z biotesti Soil samples were obtained from Slovenian industrial region Šaleska dolina and aqueous leachates were prepared. The toxicity and genotoxicity potential of these aqueous fractions have been evaluated by in vitro toxicity and genotoxicity bioassays. Freshwater toxicity test (PROTOXKIT FTM) with a ciliate protozoan Tetrahymena thermophila, and genotoxicity comet assay using Tetrahymena thermophila, Caco-2 and HepG2 cell lines were performed. Biological data were completed through chemical analyses. It has been shown that physico-chemical analyses alone may not be sufficient to characterize soil hazards. To study soil ecotoxcity it is therefore necessary to take into consideration both, the physico-chemical analyses, toxicity and genotoxicity assays. Na industrijskem področju Slovenije, v Šaleški dolini, smo vzorčili zemljo in pripravili vodne izlužke zemlje iz štirih vzrčnih mest. Toksični in genotoksični potencial vodnih izlužkov smo ovrednotili z uporabo in vitro biotestov za toksičnost in genotoksičnost. Izvedli smo test toksičnosti s sladkovodno praživaljo, migetalkarjem, Tetrahymena thermophila, s t.i. PROTOXKIT FTM testom in test genotoksičnosti: kometni test s praživaljo Tetrahymena thermophila, in Caco-2 ter HepG2 celičnima linijama. Rezultate biotestov smo dopolnili s fizikalno-kemijskimi analizami. Za relevantnejše ekotoksikološko vrednotenje vzorcev zemlje je pomembno združiti fizikalno-kemijske analize in teste toksičnosti in genotoksičnosti. microbiology toxicology genotoxicology soil samples in vitro bioassays Slovenia mikrobiologija genotoksičnost toksičnost vzorce zemlje in vitro biotesti Slovenija true false false Biotehniška fakulteta Angleški jezik Slovenski jezik Delo ni kategorizirano 2015-07-10 18:50:29 2015-07-10 18:50:29 2022-07-05 03:42:48 0000-00-00 00:00:00 2005 0 0 Str. 27-38 št. 1 Letn. 86 2005 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 1581-9175 1813384 213840640 http://www.dlib.si/details/URN:NBN:SI:doc-IWLTLBX7 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=60787 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0