20.500.12556/RUL-59608 Sposobnost biohidrogenacije linolne kisline pri vampni bakteriji Pseudobutyrivibrio xylanivorans Mz5T Capability of biohydrogenation of linoleic acid in rumen bacterium Pseudobutyrivibrio xylanivorans Mz5T Konjugirane linolne kisline (CLA) so skupina pozicijskih in geometrijskih izomer oktadekadienojske kisline (18:2) in imajo vrsto ugodnih učinkov pri ljudeh in živalih. Glavni tvorci CLA so vampne bakterije, predvsem iz rodu Butyrivibrio. Vse več je raziskav, s katerimi se preiskušajo načini za povečanje vsebnosti CLA v prehranskih proizvodih živalskega izvora, kar bi lahko dosegli z uporabo primernih probiotičnih sevov bakterij. Eden izmed njihje tudi Pseudobutyrivibrio xylanivorans Mz5T. Ta sev je sposoben rasti v gojišču, kjer je prisotna linolna kislina (LA, to je prekurzor CLA) vsaj do koncentracije 200 g ml-1. Pri takih pogojih se rast sicer upočasni, a bakterije še vedno ohranijo svojo aktivnost. Po 24 urah gojenja v gojišču z LA proučevani sev izvede biohidrogenacijo LA do trans-vakcenske kisline, ki jo tkivni encim ?9-desaturaza, prisoten v tkivu živali, lahko pretvori nazaj do CLA. Z uporabo seva Mz5T kot probiotika v prehrani živali bi tako lahko povečali kakovost končnih živil živalskega izvora. Conjugated linoleic acids (CLA) are positional and geometrical isomers of octadecadienoic acid (18:2) and have a variety of beneficial effects for the humans and animals. Main producers of CLA are rumen bacteria, mainly from the genus Butyrivibrio. Many researches are directed towards increasing the concentration of CLA in food products of animal origin. This could be achieved also with the application of suitable probiotic strains of bacteria. One of those is also Pseudobutzrivibrio xzlanivorans My5T. This strain is capable to grow in the presence of linoleic acid (LA, ie. the CLA precursor), at least up to concentrations of 200 g LA/ml. Under these conditions, the lag phase is prolonged, and the growth is slowered down, too. Consequently the bacteria retain their activity. After 24 hour incubation in the medium with LA, the studied strain biohydrogenates the LA to trans-vaccenic acid, which can be then transformed back to CLA by tissue ?9-desaturase, which is present in the animal tissue. The strain Mz5T could be used as a probiotic in animal nutrition in order to increase the quality of the food products of animal origin. mikrobiologija anaerobne bakterije Pseudobutyrivibrio xylanivorans konjugirana linolna kislina biohidrogenacija vamp microbiology anaerobic bacteria Pseudobutyrivibrio xylanivorans conjugated linoleic acid biohydrogenation rumen true false false Biotehniška fakulteta Slovenski jezik Angleški jezik Delo ni kategorizirano 2015-07-10 18:29:30 2015-07-10 18:29:30 2022-07-05 03:42:00 0000-00-00 00:00:00 2006 0 0 Str. 75-81 št. 2 Letn. 88 2006 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 1581-9175 1969032 213840640 http://www.dlib.si/details/URN:NBN:SI:doc-6UM44NT1 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=59619 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0