20.500.12556/RUL-36986 Membrane changes associated with exposure of Pseudomonas putida to selected environmental pollutants and their possible roles in toxicity A bacterial model system (Pseudomonas putida DSM 50026) was used in this research to assess potential effect of five selected chemically diverse environmental pollutants on cell membranes. Long chain fatty acid profiles of cultures exposed to environmentally relevant concentrations of atrazine (ATR), metolachlor (MET), pentachlorobiphenyl (PCB), hexachlorobenzene (HCB) and fluoranthene (FL), were analyzed and compared to non0exposed cultures. To assess sensitivity of membrane-based response, the impact of each toxicant on culture growth was also followed spectrophotometrically. Results revealed changes in fatty acid profiles when cells were exposed to PCB, HCB and FL in concentrations below the inhibitory levels. Moreover, the observed membrane responses were similar to the ones previously associated with adaption to some membrane-active compounds. On the other hand, exposure of cells to any of the two herbicides, ART or MET, did not induce any significant changes in fatty acid profiles. However, when combined with a commonly used fertilizer compound, NH[down]4NO[down]3, growth impairment was observed. Synergisitc effect of the two herbicides with NH[down]4NO[down]3, might be a consequence of changes in fatty acid profile increasing membrane fluidity, likely induced by NH[down]4+ ions. mikrobiologija toksikologija bakterije Pseudomonas putida celične membrane toksične snovi true false false Angleški jezik Angleški jezik Delo ni kategorizirano 2015-07-10 12:55:53 2015-07-10 12:55:53 2022-06-23 03:51:20 0000-00-00 00:00:00 2012 0 0 Str. 83-88 no. 1 Vol. 59 2012 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579:632.95.024 1318-0207 3024008 14086149 http://acta.chem-soc.si/59/59-1-83.pdf 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=36997 Biotehniška fakulteta 0 0 0