20.500.12556/RUL-36560 Bioinformatic evidence and characterization of novel putative large conjugative transposons residing in genomes of genera Bacteroides and Prevotella Bioinformatic evidence of the presence of a large conjugative transposon in ruminal bacterium Prevotella bryantii B[sub]14[sup]T is presented. The described transposon appears to be related to another large conjugative transposon CTnBST, described in Bacteroides uniformis WH207 and to the conjugative transposon CTn3-Bf, which was observed in the genome of Bacteroides fragilis strain YCH46. All three transposons share tra gene regions with high amino acid identity and clearly conserved gene order. Additionally, a second conserved region consisting of hypothetical genes was discovered in all three transposons and named the GG region. This region served as a specific sequence signature and made possible the discovery of several other apparently related hypothetical conjugative transposons in bacteria from the genus Bacteroides. A cluster of genes involved in sugar utilization and metabolism was discovered within the hypothetical CTnB[sub]14, to a certain extent resembling the polysaccharide utilization loci which were described recently in some Bacteroides strains. This is the first firm report on the presence of a large mobile genetic element in any strain from the genus Prevotella. mikrobiologija Bacteroides Prevotella molekularna genetika genom bioinformatika true false false Angleški jezik Angleški jezik Delo ni kategorizirano 2015-07-10 12:30:30 2015-07-10 12:30:30 2022-06-23 03:49:53 0000-00-00 00:00:00 2012 0 0 Str. 285-290 no. 4 Vol. 57 2012 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579:575 0015-5632 10.1007/s12223-012-0126-5 3042440 25470976 http://www.springerlink.com/content/rm17547259520w21/fulltext.pdf 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=36571 Biotehniška fakulteta 0 0 0