20.500.12556/RUL-36545 Demonstration of neuraminidase activity in Mycoplasma neurolyticum and of neuraminidase proteins in three canine Mycoplasma species Neuraminidases are virulence factors in many pathogenic microarganisms. They are present also in some Mycoplasma species that cause disease in birds, dogs and alligators. Thirty-seven Mycoplasma species have been examined previously for neuraminidase (sialidase) activity, whereas many of the species causing disease in man, ruminants, pigs, rodent and other animals have not. In this study neuraminidase enzymatic activity (NEAC) was examined in 45 previously untested Mycoplasma species, including those causing diseases in man, farm animals and laboratory animals. The only species in which NEAC was found was Mycoplasma neurolyticum, specifically, its type strain (Type A[up]T) which is capable of inducing neurologic signs in inoculated young mice and rats. The NEAC of washed cells was relatively weak, but it differed even more than 10-fold among cells of cultures derived from individual colonies of M. neurolyticum. A weak NEAC was also detected in the supernatant of the M. neurolyticum broth culture. Canine Mycoplasma spp. with high sialidase activity reported previously, Mycoplasma canis, Mycoplasma cynos and Mycoplasma molare had 100-fold more NEAC than M. neurolyticum, but apparent differences in NEAC levels existed among strains of M. canis and of M. cynos. Zymograms using neuraminidase-specific chromogenic substrate were used to showproteins havingNEAC. In M. canis (a field isolate Larissa and the type strain PG14[up]), M. cynos (isolate 896) and M. molare (type strain H542[up]) proteins with NEAC had molecular mases of - 130 kDA, 105 kDa and 110 kDa, respectively. Identification of these neraminidases could provide the basis for their molecular characterization. mikrobiologija mikoplazme Mycoplasma neurolyticum nevraminidaze true false false Angleški jezik Angleški jezik Delo ni kategorizirano 2015-07-10 12:30:19 2015-07-10 12:30:19 2022-06-23 03:49:48 0000-00-00 00:00:00 2012 0 0 Str. 425-429 no. 2/4 Vol. 155 2012 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 0378-1135 10.1016/j.vetmic.2011.08.026 2958984 1191700 http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271229&_user=4776866&_pii=S037811351100472X&_check=y&_origin=browse&_zone=rslt_list_item&_coverDate=2012-03-23&wchp=dGLzVlk-zSkWb&md5=1bfd4b5e968171ab003546dea1739912/1-s2.0-S037811351100472X-main.pdf 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=36556 Biotehniška fakulteta 0 0 0