20.500.12556/RUL-36542 Inability of Prevotella bryantii to form a functional Shine-Dalgarno interaction reflects unique evolution of ribosome binding sites in Bacteroidetes The Shine-Dalarno (SD) sequence is a key element directing the translation to initiate at the authentic start codons and also enabling translation initiation to proceed in 5' untranslated mRNA regions (5'-UTRs) containing moderately strong secondary structures. Bioinformatic analysis of almost forty genomes from the major bacterial phylum Bacteroidetes revealed, however, a general absence of SD sequence, drop in GC content and consequently reduced tendency to form secondary structures in 5'-UTRs. The experimentsusing the Prevotella bryantii TC-11 expression system were in agreement with this findings: neither addition nor omission of SD sequence in the unstructured 5'-UTR affected the level of the reporter protein, non-specific nuclease NucB. Further, NucB level in P. bryantii TC1-1, contrary to hMGFP level in Eschericihia coli, was five times lower when SD sequence formed part of the secondary structure with a folding energy -5,2 kcal/mol. Also, the extended SDsequences did not affect protein levels as in E. coli. It seems therefore that a functional SD interaction does not take place during the translation initiation in P. bryantii TC1-1 and possibly other members of phylum Bacteroidetes although the anti SD sequence is present in 16S rnRNA genes of their genomes. We thus propose that in the absence of the SD sequence interaction, the selection of genuine start codons in Bacteroidetes is accomplished by binding of ribosomal protein S1 to unstructured 5'UTR as opposed to coding region which is inaccessible due to mRNA secondary structure. Additionally, we found that sequence logos of region preceding the start codons may be used as taxonomical markers. Depending on whether complete sequence logo or only part of it, such as information content and base proportion at specific positions, is used, bacterial genera or families and insome cases even bacterial phyla can be distinguised. mikrobiologija Bacteroidetes Prevotella bryantii molekularna genetika zaporedje Shine-Delgarno true false false Angleški jezik Neznan jezik Delo ni kategorizirano 2015-07-10 12:30:16 2015-07-10 12:30:16 2022-06-23 03:49:47 0000-00-00 00:00:00 2011 0 0 Str. 1-9, e22914 no. 8 Vol. 6 2011 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579:575 1932-6203 10.1371/journal.pone.0022914 2912136 2005896 http://www.plosone.org 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=36553 Biotehniška fakulteta 0 0 0