20.500.12556/RUL-36531 Chemiluminescence of non-differentiated THP-1 promocytes developing an assay for screening anti-inflammatory milk proteins and peptides Human leukemic THP-1 promonocytes are widely used as a model for peripheral blood monocytes. However, superoxide production during respiratory burst (RB) of non-differentiated THP-1 (nd-THP-1) cells is very low. Here we present a rapid and low-cost method for measuring the chemiluminescence (CL) of opsonized zymosan (OZ) induced RB which allows detection of Escherichia coli lipopolysaccharide (LPS) induced priming of nd-THP-1 cells on the basis of CL reaction kinetics. Maximum CL intensity obtained was 2.20+- 0.25 and 1.30+- 0.11 relative light units, while CL peak time was achieved at 18.1+- 2.6 and 28.7+- 1.3 min in primed and non-primed cells, respectively. The priming of nd-THP-1 cells with LPS evoked tzpical TNF-[alfa] and IL-6 production. We tested the effects of bovine lactoferrin and protein fractions from Lactobacillus helveticus BGRA43 fermented milk for potential anti-inflammatory effects on LPS primed nd-THP-1 cells. Four fractions were found to inhibit the OZ-induced CL in a dose-dependent manner (IC50 3-30 g/mL), while lactoferrin inhibited CL to a lesser extent (IC50 270? g/mL). These results suggest that measuring CL response of nd-THP-1 cells can serve as a method for screening anti-inflammatory compounds which could be used in reducing the risk of phagocyte-mediated inflammatory diseases. mleko beljakovine peptidi analizna kemija kemiluminiscenca true false false Angleški jezik Neznan jezik Delo ni kategorizirano 2015-07-10 12:30:08 2015-07-10 12:30:08 2022-06-23 03:49:43 0000-00-00 00:00:00 2011 0 0 Str. 251-258 no. 4 Vol. 26 2011 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 637.1:543 1522-7235 10.1002/bio.1220 2757768 436108 http://onlinelibrary.wiley.com/doi/10.1002/bio.1220/abstract 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=36542 Biotehniška fakulteta 0 0 0