20.500.12556/RUL-36382 Development of new microsatellite markers for Salvia officinalis L. and its potential use in conservation-genetic studies of narrow endemic Salvia brachyodon Vandas Nine new microsatellite markers (SSR) were isolated from Salvia officinalis L. A total of 125 alleles, with 8 to 21 alleles per locus, were detected in a natural population from the east Adriatic coast. The observed heterozygosity, expected heterozygosity, and polymorphic information content ranged from 0.46 to 0.83, 0.73 to 0.93 and 0.70 to 0.92, respectively. New microsatellite markers, as well as previously published markers, were tested for cross-amplification in Salvia brachyodon Vandas, a narrow endemic species known to be present in only two localities on the Balkan Peninsula. Out of 30 microsatellite markers tested on the natural S. brachyodon population, 15 were successfully amplified. To obtain evidence of recent bottleneck events in the populations of both species, observed genetic diversity (H$_E$) was compared to the expected genetic diversity at mutation-drift equilibrium (H$_{EQ}$) and calculated from the observed number of alleles using a two-phased mutation model (TPM). Recent bottleneck events were detected only in the S. brachyodon population. This result suggests the need to reconsider the current threat category of this endemic species. salvia common sage laminaceae microsatellites microsatellite markers conservation cross-amplification Dalmatian sage genetic bottleneck Lamiaceae population genetics short-tooth sage navadni žajbelj dalmatinski žajbelj mikrosatelitski markerji mikrosateliti populacije Lamiaceae genetska pestrost true false true Angleški jezik Slovenski jezik Članek v reviji 2015-07-10 12:28:12 2015-07-10 12:28:12 2022-08-01 12:23:45 0000-00-00 00:00:00 2012 0 0 Str. 12082-12093 iss. 9 Vol. 13 2012 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2:633.88 1661-6596 10.3390/ijms130912082 7252089 36217605 RAZ_Radosavljevic_Ivan_2012.pdf RAZ_Radosavljevic_Ivan_2012.pdf 1 6E12828A29159F55D4BECC60C110A9B4 f6c72deeb48f60c3f7f678c39a651faea79cd8c56cc04b2a2985005ea45d82c1 925fc985-0be8-11ec-a523-00155dcfd717 20.500.12556/rul/0b987373-0aa9-43a5-b32e-9b5e5954e3e8 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146497 https://www.mdpi.com/1422-0067/13/9/12082 0 3ff684a0-0be8-11ec-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=146496 Biotehniška fakulteta 0 0 0