20.500.12556/RUL-32827 BEsTRF a tool for optimal resolution of terminal-restriction fragment length polymorphism (T-RFLP) analysis based on user defined primer-enzyme-sequence databases BEsTRF (Best Estimated T-RF) provides a stand-alone environment for analyzing primers-enzymes-gene section combinations used in T-RFLP for its optimal resolution. User defined sequence databases of several hundred thousand DNA sequences can be explored and the resolution of user specified sets of primers and restriction endonucleases can be analyzed on either forward or reverse terminal fragments. Sequence quality, primer mismatches, insertions and delitions can be controlled and each primer-pair specific sequence collections can be exported for downstream analyses. The configuration for a novel T-RFLP population profiling using rpoB gene (DNA-directed RNA polymerase, beta subunit) on forward fluorescently labelled primer are presented. mikrobiologija molekularna genetika BEsTRF true false false Angleški jezik Neznan jezik Delo ni kategorizirano 2015-07-10 10:57:39 2015-07-10 10:57:39 2022-06-21 03:43:49 0000-00-00 00:00:00 2009 0 0 Str. 1556-1558 Issue 12 Vol. 25 2009 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579:575 1367-4803 10.1093/bioinformatics/btp254 2450312 1410140 http://bioinformatics.oxfordjournals.org/cgi/reprint/btp254?ijkey=qw9y7y9LEcKhXd7&keytype=ref 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=32838 Biotehniška fakulteta 0 0 0