20.500.12556/RUL-190914 Vpliv izbranih dejavnikov na preživetje in proteinski profil bakterij Campylobacter jejuni v biofilmu The effect of selected factors on the survival and protein profile of Campylobacter jejuni in biofilm V doktorski disertaciji smo proučevali vpliv izbranih naravnih bioaktivnih učinkovin in medvrstnih interakcij na pritrjevanje, kultivabilnost, z virulenco povezane lastnosti ter proteinski profil bakterije Campylobacter jejuni. Ovrednotili smo učinke izbranih glivnih lektinov, monoterpenov linalola in linalil acetata ter interakcij z bakterijama Lactococcus cremoris in Pseudomonas fragi. Učinke smo proučevali na abiotskih in biotskih modelnih površinah, na celicah Caco-2 ter s kvantitativno proteomiko. Glivni lektini so površinsko odvisno zmanjšali pritrjevanje bakterije C. jejuni, izbrani lektini pa tudi njeno pritrjevanje na celice Caco-2 in invazijo vanje. Linalol in linalil acetat sta zmanjšala število kultivabilnih biofilmskih celic na polistirenu in mucinu tipa II ter invazijo v celice Caco-2, a v poskusih na zrelih biofilmskih celicah zaznane spremembe v proteinskem profilu niso bile statistično značilne. Vpliv bakterije L. cremoris je bil odvisen od površine in časa inkubacije; na polistirenu se je po 48 h zmanjšala kultivabilnost bakterije C. jejuni, na mucinu tipa II pa kultivabilnost bakterije L. cremoris. Interakcija z bakterijo P. fragi je bila dvosmerna ter odvisna od površine, temperature, atmosferskih razmer in časa inkubacije. Obe medvrstni interakciji sta povzročili obsežne statistično značilne spremembe proteinskega profila bakterije C. jejuni. Rezultati kažejo, da je odziv bakterije C. jejuni v biofilmu izrazito odvisen od površine, uporabljenega eksperimentalnega modela in okoljskih razmer ter poudarjajo pomen proučevanja večvrstnih biofilmov pri razumevanju njene obstojnosti v agroživilski verigi. In this doctoral dissertation, we investigated the effects of selected natural bioactive compounds and interspecies interactions on the adhesion, culturability, virulence-associated properties, and protein profile of Campylobacter jejuni. We evaluated the effects of selected fungal lectins, the monoterpenes linalool and linalyl acetate, and interactions with Lactococcus cremoris and Pseudomonas fragi. The effects were studied on abiotic and biotic model surfaces, Caco-2 cells, and using quantitative proteomics. Fungal lectins reduced C. jejuni adhesion in a surface-dependent manner, while selected lectins also reduced its adhesion to and invasion of Caco-2 cells. Linalool and linalyl acetate reduced the number of culturable biofilm cells on polystyrene and mucin type II and decreased invasion of Caco-2 cells, but observed changes in the protein profile of mature biofilm cells were not statistically significant. The effect of L. cremoris depended on the surface and incubation time; after 48 h, the culturability of C. jejuni decreased on polystyrene, whereas the culturability of L. cremoris decreased on mucin type II. The interaction with P. fragi was bidirectional and depended on the surface, temperature, atmospheric conditions, and incubation time. Both interspecies interactions caused extensive statistically significant changes in the protein profile of C. jejuni. The results show that the response of C. jejuni in biofilms strongly depends on the surface, the experimental model, and environmental conditions, highlighting the importance of studying multispecies biofilms for understanding its persistence throughout the agrifood chain. Campylobacter jejuni biofilmi lektini monoterpeni Lactococcus cremoris Pseudomonas fragi enovrstni biofilmi večvrstni biofilmi kvantitativna proteomika Campylobacter jejuni biofilms lectins monoterpenes Lactococcus cremoris Pseudomonas fragi monospecies biofilms multispecies biofilms quantitative proteomics true false false [B. Jug] Slovenski jezik Angleški jezik Doktorsko delo/naloga 2026-11-07 07:15:12 2026-11-07 07:15:40 2026-11-09 08:10:35 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.22/.26:579.67:577.2 303032 304359939 64933.pdf 64933.pdf 1 EBD994FD3E2D6A203238BAABD9BA05E5 e30452e80e74547d71dbcfd672a24060c59e8623bafd08d5eff4502c0ac7f714 935bf6e9-da72-11f1-aea2-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=252716 Biotehniška fakulteta 0 0 0