20.500.12556/RUL-190790 Association of target miRNAs expression in blood plasma and cerebrospinal fluid with Alzheimer's disease biomarkers level and cognitive decline Epigenetic changes can affect Alzheimer's disease (AD) susceptibility. miRNAs are novel potential circulating biomarkers of AD and mild cognitive impairment (MCI) that could support cerebrospinal fluid (CSF) biomarkers in earlier diagnosis of the disease or determination of disease stage. Our aim was to assess differences in expression of target miRNAs in patients with different stages of cognitive impairment and to evaluate the association with CSF biomarkers or cognitive test score (MMSE). We included 117 patients with cognitive impairment, among them 62 AD patients, 24 MCI patients with pathological CSF biomarker levels, and 31 MCI patients with normal CSF biomarker levels. Expression of seven target miRNAs was measured in patients' blood plasma, CSF and extracellular vesicles (EVs) enriched from plasma and CSF. None of the investigated miRNAs were differentially expressed between AD and MCI groups. Four miRNAs were associated with CSF biomarker levels, both in plasma (hsa-miR-375-3p) and CSF (hsa-miR-146a-5p, hsa-miR-29c, hsa-miR-107). The observed findings suggest that investigated miRNAs are not suitable for differentiation between different stages of cognitive impairment. However, miRNAs were associated with typical hallmarks of AD, which supports their important role in neurodegeneration. Therefore, miRNA regulatory networks could contribute to better understanding of the biological processes involved in cognitive impairment. Alzheimer’s disease biomarker extracellular vesicles mild cognitive impairment miRNA Alzheimerjeva bolezen biološki označevalec zunajcelični vezikli blaga kognitivna motnja mikro RNA true false true Angleški jezik Slovenski jezik Članek v reviji 2026-11-04 09:25:51 2026-11-04 09:25:57 2026-11-06 04:29:20 0000-00-00 00:00:00 2026 0 0 17 str. art. 34202 Vol. 16 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2:616.8 2045-2322 10.1038/s41598-026-63369-3 287673347 RAZ_Vogrinc_David_2026.pdf RAZ_Vogrinc_David_2026.pdf 1 7593C8A7AC1A2EDD76DA843E469CB2A7 72afa6ca74f1d68e14bebe7f9a204eb6a853fd2ec4626843c8783b20566208e9 1cb089b7-d82a-11f1-aea2-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=252431 https://www.nature.com/articles/s41598-026-63369-3 1 4d4eb694-d829-11f1-aea2-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=252429 Medicinska fakulteta 0 0 0