20.500.12556/RUL-190428 Bioash amendments from biomass energy systems reshape rhizosphere bacterial communities and reduce metal bioavailability in contaminated soils Biomass-derived ashes (bioash) from energy production are increasingly promoted within circular bioeconomy frameworks as soil amendments that recycle nutrients and mitigate soil acidity. However, their impacts on rhizosphere bacterial communities in metal-contaminated soils (MCS) remain poorly understood. Here, we investigated how four bioash types applied at three rates influence rhizosphere biogeochemistry and bacterial community dynamics of lettuce (Lactuca sativa L.) grown in MCS. Bioash amendment consistently increased soil pH and electrical conductivity, while significantly decreasing bioavailable Zn and Cd fractions. High-throughput 16S rRNA gene amplicon sequencing revealed limited effects on bacterial alpha diversity, with only slight increases at the highest bioash doses. In contrast, beta diversity analyses demonstrated pronounced, dose- and type-dependent shifts in community composition (PERMANOVA, R² = 0.46, p = 0.009). Multivariate analyses identified rhizosphere pH, salinity, DTPA-extractable K and Cd, and mineral N as the main physicochemical variables associated with bacterial community composition. At the phylum level, bioash application increased the relative abundance of Pseudomonadota, while the relative abundances of Bacillota, Bacteroidota, and Gemmatimonadota declined under high application rates. Differential abundance analysis highlighted a distinct subset of bioash-responsive taxa, with enrichment of genera such as Galbitalea, Dongia, and Caulobacter, alongside depletion of several putatively metal-sensitive groups. Overall, bioashes reduced metal bioavailability and restructured rhizosphere microbiomes, supporting their role in sustainable remediation, while emphasizing the need to optimize application rates to balance soil recovery and microbial ecological integrity. biomass-derived ashes soil metal contamination 16S rRNA gene amplicon sequencing rhizosphere bacterial community pepel iz biomase onesnaženje tal s kovinami sekvenciranje bakterijske združbe rizosfera true false true Angleški jezik Slovenski jezik Članek v reviji 2026-10-28 15:05:50 2026-10-28 15:05:58 2026-10-29 04:05:57 0000-00-00 00:00:00 2026 0 0 14 str. art. 100872 Vol. 24 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 631.4 2772-4271 10.1016/j.nexus.2026.100872 302937859 Energy_Nexus_(1-s2.0-S2772427126002445-main)_(003).pdf Energy_Nexus_(1-s2.0-S2772427126002445-main)_(003).pdf 1 B837CBD534D5A8CC915ACC4C3519B5F0 3c66b8132b2356d0594d06e2fd3c5299619ffeb5b62474e1cda2b43dc57bfe2c df5cfe10-d2d8-11f1-aea2-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=251758 https://www.sciencedirect.com/science/article/pii/S2772427126002445 1 af5c5e58-d2d8-11f1-aea2-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=251757 Biotehniška fakulteta 0 0 0