20.500.12556/RUL-190027 Razvoj in uporaba elektrokemijskih senzorjev za določanje izbranih organskih onesnaževal raziskovalni podatki, obravnavani v doktorskem delu Development and applications of electrochemical sensors for the determination of selected organic pollutants research data underlying the doctoral dissertation Raziskovalno delo obravnava razvoj elektrokemijskih senzorjev za določanje izbranih organskih onesnaževal kot alternative konvencionalnim analiznim metodam, ki pogosto zahtevajo drago opremo, usposobljeno osebje, dolgotrajne postopke ter večjo porabo topil in reagentov. Razviti so bili trije senzorski sistemi za določanje elektrokemijsko aktivnih neonikotinoidov, elektrokemijsko neaktivnih neonikotinoidov in polistirenske nanoplastike. Prvi senzor je bil razvit za določanje klotianidina, imidakloprida in tiametoksama na sitotiskanih trielektrodnih sistemih. Površina delovne elektrode je bila modificirana s preprostim, hitrim in ponovljivim elektrokemijskim predkondicioniranjem. Pri optimizaciji postopka je bil testiran vpliv različnih elektrolitov ter izbrane elektrokemijske metode. Elektrokemijska in površinska karakterizacija sta potrdili povečanje elektrokemijsko aktivne površine, izboljšanje prenosa naboja, odstranitev površinske kontaminacije in povečanje vsebnosti kisikovih funkcionalnih skupin. Po optimizaciji merilnih pogojev so bile z adsorpcijsko diferenčno pulzno voltametrijo določene umeritvene premice in meje zaznave. Ovrednoteni so bili stabilnost senzorja, ponovljivost priprave in vplivi potencialnih interferentov. Uporabnost senzorja je bila preverjena v vzorcih površinske vode in medu. Drugi senzor je bil namenjen določanju acetamiprida in tiakloprida z uporabo molekularno vtisnjenih polimerov kot prepoznavnih elementov. Optimizirani so bili pogoji sinteze, izbira monomerov ter razmerja med analitom, monomerom in zamreževalcem. Polimeri so bili karakterizirani z vrstično elektronsko mikroskopijo in infrardečo spektroskopijo, njihove vezavne lastnosti pa ovrednotene z adsorpcijskimi in kinetičnimi raziskavami. Na podlagi faktorjev vtisnjenja so bili izbrani najprimernejši polimeri in optimiziran njihov nanos na elektrodo iz steklastega ogljika. Za oba analita sta bili določeni umeritveni premici in preverjena selektivnost sistema. Tretji senzor je bil razvit za določanje polistirenske nanoplastike. Zlata delovna elektroda sitotiskanega trielektrodnega sistema je bila modificirana s tanko plastjo mezoporozne silike in dodatno funkcionalizirana s prolinom. Senzor je bil karakteriziran z mikroskopskimi, spektroskopskimi in elektrokemijskimi metodami. Preučevani so bili polistirenski delci treh velikosti, katerih lastnosti so bile ovrednotene z dinamičnim sipanjem svetlobe, meritvami zeta potenciala in vrstično elektronsko mikroskopijo. Optimizirana sta bila potencial in čas akumulacije delcev na površini senzorja. Umeritvene premice so bile določene z diferenčno pulzno voltametrijo in elektrokemijsko impedančno spektroskopijo. Največja občutljivost je bila dosežena za najmanjše delce, z večanjem njihove velikosti pa se je zmanjševala. Uporabnost senzorja je bila preverjena tudi v vzorcu ustekleničene vode. Raziskovalni podatki vključujejo rezultate priprave, optimizacije, karakterizacije in vrednotenja razvitih senzorskih sistemov ter podpirajo nadaljnji razvoj elektrokemijskih pristopov za spremljanje organskih onesnaževal. This research addresses the development of electrochemical sensors for the determination of selected organic pollutants as an alternative to conventional analytical methods, which often require expensive equipment, trained personnel, time-consuming procedures, and substantial consumption of solvents and reagents. Three sensing systems were developed for the determination of electrochemically active neonicotinoids, electrochemically inactive neonicotinoids, and polystyrene nanoplastics. The first sensor was developed for the determination of clothianidin, imidacloprid, and thiamethoxam using screen-printed three-electrode systems. The working electrode surface was modified through a simple, rapid, and repeatable electrochemical preconditioning procedure. The effects of different electrolytes and the selected electrochemical method were investigated during procedure optimisation. Electrochemical and surface characterisation confirmed an increase in the electrochemically active surface area, enhanced charge transfer, removal of surface contamination, and an increase in the abundance of oxygen-containing functional groups. Following optimisation of the measurement conditions, calibration curves and limits of detection were established using adsorptive differential pulse voltammetry. Sensor stability, preparation repeatability, and the effects of potential interferents were evaluated. The applicability of the sensor was assessed in surface water and honey samples. The second sensor was designed for the determination of acetamiprid and thiacloprid using molecularly imprinted polymers as recognition elements. Synthesis conditions, monomer selection, and the ratios of analyte, monomer, and cross-linker were optimised. The polymers were characterised using scanning electron microscopy and infrared spectroscopy, while their binding properties were evaluated through adsorption and kinetic studies. The most suitable polymers were selected based on their imprinting factors, and their deposition onto a glassy carbon electrode was optimised. Calibration curves were established for both analytes, and the selectivity of the system was assessed. The third sensor was developed for the determination of polystyrene nanoplastics. The gold working electrode of a screen-printed three-electrode system was modified with a thin mesoporous silica film and further functionalised with proline. The sensor was characterised using microscopic, spectroscopic, and electrochemical methods. Polystyrene particles of three sizes were investigated, and their properties were evaluated using dynamic light scattering, zeta potential measurements, and scanning electron microscopy. The potential and duration of particle accumulation on the sensor surface were optimised. Calibration curves were established using differential pulse voltammetry and electrochemical impedance spectroscopy. The highest sensitivity was achieved for the smallest particles, with sensitivity decreasing as particle size increased. The applicability of the sensor was also assessed in a bottled water sample. The research data include the results of the preparation, optimisation, characterisation, and evaluation of the developed sensing systems and support the further development of electrochemical approaches for monitoring organic pollutants. elektrokemijski senzorji voltametrija neonikotinoidi polistirenska nanoplastika electrochemical sensors voltammetry neonicotinoids polystyrene nanoplastics true false false Slovenski jezik Angleški jezik Raziskovalni podatki 2026-10-20 18:51:47 2026-10-22 16:00:21 2026-10-23 04:11:39 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2027-10-20 README.txt README.txt README 1 2D75955E82E21921D885D5A172D2563D abb80668df440296bec2d7ee7769777c427077e8392bfb59547776ec4df2809e 875e2e4c-ccb0-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=251015 Hipoteza_1.zip Hipoteza_1.zip Razvoj senzorja za detekcijo elektrokemijsko aktivnih neonikotinoidov (prvi del) 1 8E28733E6A36DD3FE83AA950A90AAB08 fef2e7bc755355d1ff2f2a7b548ba4a1b9e5be910cefbe07707f40c0bea77291 b7976b8a-ccaa-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=251011 Hipoteza_2.zip Hipoteza_2.zip Razvoj senzorja za detekcijo elektrokemijsko aktivnih neonikotinoidov (drugi del) 1 31942B3E3BB9405193594FBD6D55877A 4a79d21d4e90d63e7c44fc2cb3ab1d8e51031517a7cd617332c5f2f8851a4eef b85167c9-ccaa-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=251014 Hipoteza_3.zip Hipoteza_3.zip Razvoj senzorja za detekcijo elektrokemijsko neaktivnih neonikotinoidov 1 0785057B4166FCE821B82927FCCB139B 80624bfdab1034540e935d08ed97cf0ca95f212b11f69e18bff30550c2858031 b80dccb2-ccaa-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=251012 Hipoteza_4.zip Hipoteza_4.zip Razvoj senzorja za detekcijo polistirenske nanoplastike 1 AC5C70C4F6915E1AD8255EEE6689D1B1 c2de426440095fb0d5e9ea7f6e49d97db006884c1be7d163eeb8f73a29991fa8 b8305539-ccaa-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=251013 Fakulteta za kemijo in kemijsko tehnologijo Poskus: Laboratorij 0 0 0