20.500.12556/RUL-189748 Season-long lateral-shoot removal alters fruit volatiles and glucose content in primocane-fruiting raspberry (Rubus idaeus L. cv. ‘Enrosadira’) research data underlying the article Pruning is an essential horticultural practice used to shape raspberry plant architecture, with consequent effects on the balance between vegetative growth and fruit production. In this study, we investigate for the first time the effects of removing lateral shoots in the primocane-fruiting raspberry cultivar ‘Enrosadira’. In branched plants, lateral shoots were retained, whereas in unbranched plants laterals were removed throughout the season. We evaluated yield, vegetative plant parameters (height, leaf surface area, number of leaflets, net photosynthesis), and targeted primary-metabolite and volatile profiling (sugars, organic acids, ascorbic acid, phenolic compounds and volatile organic compounds), using HPLC and GC-MS. Glucose content was up to 45% higher in fruits from branched plants right before the seasonal peak. Statistically higher values were observed in the fruits from the unbranched treatment for total aldehydes compared to the branched treatment. Furthermore, several individual volatiles were significantly higher in the unbranched treatment, including five aldehydes (hexanal, hex-3-enal, hex-(2E)-enal, decanal, and dodecanal), three monoterpenes (α-pinene, α-phellandrene, and β-phellandrene), octanoic acid, and α-ionol, a key contributor to raspberry aroma. Under the conditions tested, lateral-shoot removal did not affect yield or the measured vegetative traits but altered selected key quality fruit metabolites of primocane-fruiting raspberry cultivar ‘Enrosadira’. pruning lateral shoots canopy architecture quality obrezovanje stranski poganjki arhitektura krošnje kakovost true false false Angleški jezik Slovenski jezik The present study was conducted in the experimental field of the Biotechnical Faculty of the University of Ljubljana, Slovenia (latitude: 46°02’ N; longitude: 14°28’ E; altitude: 295 m a.s.l.). The present study on the seasonal removal of lateral shoots on primocane-fruiting raspberry (Rubus idaeus L.) was conducted from June to November 2025. Raziskovalni podatki 2026-10-14 13:10:30 2026-10-16 12:40:01 2026-10-17 04:16:13 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 Materials_and_methods.docx Materials_and_methods.docx Materials and methods 1 6ED7DD8A211A046222B0B9BC9C165AF5 f476e3e44268ed245b93ee58f786c3d8bfce541987a16424650cdb99433cb784 97502d3d-c7df-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=250543 Original_data.xlsx Original_data.xlsx Original data 1 52296ADD19557696216161ECB398770D ff4e3c5656a8c75a4d432b4b51b35a6422e388ff697bb07d3353d4a67a5dd3b5 0ce45485-c7df-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=250540 Biotehniška fakulteta Poskus: Teren/Intervencija 0 0 0