20.500.12556/RUL-188408 Clinical molecular investigation of Encephalitozoon cuniculi in pet rabbits early detection of potential risk for animal and human infection Background: Encephalitozoon cuniculi is an obligate intracellular parasite belonging to the phylum Microsporidia, family Encephalitozoonidae. The parasite is distributed worldwide and infects a variety of mammals, with rabbits being the main host. Currently, the most reliable diagnostic method is serological testing, but various molecular techniques are also being used. Objective: This study assesses the presence, genotypes and clinical signs of E. cuniculi infection in pet rabbits in Slovenia, using molecular and serological methods. Methods: From January 2022 to 2024, urine samples from random pet rabbits (n = 247) were collected for polymerase chain reaction (PCR) testing and transmission electron microscopy (TEM) to search for the presence of E. cuniculi genetic material or spores. Results: With the real-time PCR, E. cuniculi genetic material was detected in 37 of 247 (14.98%) rabbit urine samples. Serological testing revealed specific immunoglobulin M (IgM) and/or immunoglobulin G (IgG) antibodies in 91 of 133 rabbits (68.42%). A quarter of the serologically positive rabbits (25.27%) and almost half of the molecularly positive rabbits (43.24%) were clinically healthy and showed no signs of encephalitozoonosis. E. cuniculi genetic material in urine was mostly discovered in rabbits with signs of urinary tract disorders (27.03%) or neurological clinical signs (21.62%). Genotype I of E. cuniculi was detected in all rabbits. Despite the detection of the pathogens’ DNA, no E. cuniculi spores were observed by TEM. Conclusion: E. cuniculi infection is common among pet rabbits in Slovenia, with a high seroprevalence and detectable urinary shedding of parasite DNA. The presence of clinically healthy but infected rabbits indicates that subclinical infections are frequent. Genotype I appears to be the predominant genotype in the studied population. Combined molecular and serological testing may improve the detection and epidemiological characterization of E. cuniculi infections in rabbits. clinical signs Encephalitozoon cuniculi genotype molecular detection pet rabbit urine klinični znaki Encephalitozoon cuniculi genotip molekularno dokazovanje hišni kunec urin true false true Angleški jezik Slovenski jezik Članek v reviji 2026-09-23 04:08:26 2026-09-23 09:11:02 2026-09-24 04:25:02 0000-00-00 00:00:00 2026 0 0 9 str. no. 5, art. e71081 Vol. 12 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616.9:636.92 2053-1095 10.1002/vms3.71081 291605763 RAZ_Skrbec_Marusa_2026.pdf RAZ_Skrbec_Marusa_2026.pdf 1 E596E9965B56564A301071A79CABF5E5 d1ba43367dc0ef66179fdd5b16f8e8b37017895bbdd0e0674ef669582c2d1b04 0922a3e3-b71e-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=248650 https://onlinelibrary.wiley.com/doi/10.1002/vms3.71081 1 9ee98ccc-b6f3-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=248581 Veterinarska fakulteta Medicinska fakulteta 0 0 0