20.500.12556/RUL-188213 Optimizacija enkapsulacije bakteriofagov z alginatom in liofilizacijo Development of bacteriophage alginate encapsulation followed by lyophilization Bakteriofagi predstavljajo obetavno alternativo antibiotikom, zlasti zaradi naraščajoče protimikrobne odpornosti, vendar njihovo uporabo omejuje predvsem nestabilnost v kislem okolju prebavil in med shranjevanjem. V okviru magistrskega dela smo zato razvili in optimizirali postopek priprave bakteriofagov, ki vključuje namnoževanje, koncentriranje s tangencialno pretočno filtracijo, enkapsulacijo v alginatne kapsule ter liofilizacijo. Preučevali smo vpliv različnih procesnih parametrov enkapsulacije kot je koncentracija alginata, koncentracije CaCl₂ raztopine ter hitrost mešanja, na učinkovitost priprave in lastnosti končnega produkta. Testirali smo različne pogoje enkapsulacije ter demonstrirali, da kombinacija 2 % alginata, 0,1 M CaCl₂ in dodatka trehaloze omogoča formulacijo stabilnih kapsul z visoko učinkovitostjo enkapsulacije. Spreminjanje procesnih parametrov je vplivalo na morfologijo in poroznost kapsul ter posledično na sproščanje bakteriofagov iz kapsul. Demonstrirali smo, da enkapsulacija in liofilizacija bistveno izboljšata stabilnost bakteriofagov v kislem okolju, kar potrjuje uporabnost razvitega sistema za peroralno dostavo. Bacteriophages represent a promising alternative to antibiotics, particularly due to the increasing antimicrobial resistance; however, their application is mainly limited by their instability in the acidic environment of the gastrointestinal tract and during storage. In this master's thesis, we developed and optimized a process for bacteriophage formulation, including propagation, concentration by tangential flow filtration, encapsulation in alginate capsules, and lyophilization. We investigated the influence of various encapsulation process parameters, such as alginate concentration, concentration of CaCl₂, and mixing rate on properties of final formulation. Different encapsulation conditions were tested, demonstrating that a combination of 2% alginate, 0.1 M CaCl₂, and the addition of trehalose enables the formation of stable capsules with high encapsulation efficiency. Variation of process parameters affected capsule morphology and porosity, and consequently bacteriophage release. We demonstrated that encapsulation and lyophilization significantly improve bacteriophage stability in acidic conditions, thus confirming aplicability of the developed formulation procedure for oral delivery. bakteriofagi enkapsulacija alginat liofilizacija bakteriofagna terapija stabilnost bacteriophage encapsulation alginate lyophylization bacteriophage therapy stability true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-09-19 08:16:54 2026-09-19 08:16:55 2026-09-23 12:27:28 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 290075 292177923 61431.pdf 61431.pdf 1 CE938B3A0BEB91D465BEDF50885FACB3 d6cb7d426af2520c0d294c9bfb1567d94d902f4e40c7c84866427f01bd3a391e a869e1aa-b3f1-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=248242 Biotehniška fakulteta 0 0 0