20.500.12556/RUL-188022 The influence of slope calculation method on the reliability of near-infrared spectroscopy parameters during a vascular occlusion test research data underlying the article Aim: To assess within and between-day reliability of near-infrared spectroscopy derived measures of microvascular function during a vascular occlusion test (VOT), to assess whether different methods for slope calculation and blood volume correction improve reliability. Methods: Twenty-three healthy adults (11 males, 12 females) completed three laboratory visits, during which a VOT was performed using a continuous-wave NIRS device on the forearm. Reoxygenation (reoxyslope) and deoxygenation (deoxyslope) slopes along with muscle oxygen uptake (mVO2) were calculated using a fixed 10-s average, as well as maximal 3- and 1-s rolling averages for reoxygenation and average, 30-s and 10-s rolling average for deoxygenation parameters. Additionally, mVO2 was corrected for blood volume change (mVO2corr). Intraclass correlation coefficients were used to determine the within- and between-day reliability of selected measures. Results: There were no differences in within- or between-day reliability for different methods of calculation of selected parameters. However, significantly higher values were observed when shorter time window was used. In general, the parameters exhibited moderate to good within- and poor to moderate between-day reliability. Blood volume correction improved reliability of mVO2 values. Linear mixed model analysis revealed sex as a significant predictor of reoxyslope and mVO2corr, with males exhibiting slower reoxyslope and higher mVO2corr. Conclusion: The slope calculation method significantly influences the values obtained during VOT, while their reliability remains unchanged. The use of a blood volume correction is warranted and data quality reporting in future studies seems warranted. Significant sex differences were observed, highlighting the different mechanism regulating microvascular function during VOT. near-infrared spectroscopy vascular occlusion test reliability microvascular reactivity bližnje infrardeča spektroskopija test žilne okluzije zanesljivost mikrovaskularna reaktivnost true false false Angleški jezik Slovenski jezik Neznano 2026-09-17 13:40:26 2026-09-17 15:56:28 2026-09-18 04:30:46 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2027-01-06 README.txt README.txt README 1 A87AA43DCEEBE6939E27A0489C8B5F97 6c6c93ab158353765648e1140bf835f71924b63e6f7d95f19573d761ccdd410e eeb5e124-b291-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247972 Data_NIRSVOT.xlsx Data_NIRSVOT.xlsx Data NIRSVOT 1 B1C7C4A35CF9B6D9E353E1CC12C7A870 e9ad0271ec0975a04bfdb19e776e5a48bf057ee893731a719d20fdad720605b9 f846283b-b291-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247973 Fakulteta za šport Poskus 0 0 0