20.500.12556/RUL-187998 Izdelava nanovlaken iz polikaprolaktona za podporo osteogeni diferenciaciji mezenhimskih matičnih/stromalnih celic in vitro Formulation of polycaprolactone nanofibers to support in vitro osteogenic differentiation of mesenchymal stem/stromal cells Mezenhimske matične/stromalne celice (MSC) so pomembne v tkivnem inženirstvu zaradi svoje sposobnosti samoobnavljanja in diferenciacije v različne tipe celic, med drugim osteoblaste, hondrocite in adipocite. Zaradi svojih regenerativnih lastnosti so pogosto uporabljene pri raziskavah regeneracije kostnega tkiva. Za podporo rasti, adheziji in usmerjeni diferenciaciji MSC se pogosto uporabljajo elektrostatsko sukana nanovlakna (NV). Zaradi svoje strukture posnemajo zunajcelični matriks in zagotavljajo veliko specifično površino za interakcijo s celicami. Namen magistrske naloge je bil ugotoviti, ali prisotnost NV vpliva na sposobnost osteogene diferenciacije MSC. Pripravili smo NV iz polikaprolaktona (PCL) z metodo elektrostatskega sukanja, pri čemer smo za topilo uporabili mešanico dimetilformamida (DMF) in diklorometana (DCM). Uporabili smo NV različnih debelin, katere smo uravnavali s spreminjanjem časa elektrosukanja 12-odstotne raztopine PCL. Dobljena NV smo analizirali s pomočjo optičnega in elektronskega vrstičnega mikroskopa. Na pripravljena NV smo naselili primarne MSC in ovrednotili njihovo sposobnost osteogene diferenciacije. Za primerjavo smo vključili tudi ustrezne kontrolne vzorce. Osteogeno diferenciacijo smo določali z barvanjem z alizarin rdeče S ter kvantitativnim merjenjem absorbance ekstrahiranega barvila. Ugotovili smo, da NV spodbujajo osteogeno diferenciacijo MSC, pri čemer je bil učinek izrazitejši pri večji debelini NV. Dobljeni rezultati kažejo, da lahko z ustreznim načrtovanjem lastnosti PCL NV vplivamo na osteogeno diferenciacijo MSC, kar odpira možnosti za nadaljnji razvoj in optimizacijo nosilcev iz NV za uporabo v kostnem tkivnem inženirstvu in regenerativni medicini. Mesenchymal stem/stromal cells (MSCs) are important in tissue engineering due to their capacity for self-renewal and differentiation into various cell types, including osteoblasts, chondrocytes, and adipocytes. Owing to their regenerative properties, they are frequently used in bone tissue regeneration research. Electrospun nanofibers (NFs) are commonly used to support MSC growth, adhesion, and directed differentiation. Their structure mimics the extracellular matrix and provides a large specific surface area for interactions with cells. The aim of this master’s thesis was to determine whether the presence of NFs affects the osteogenic differentiation capacity of MSCs. Polycaprolactone (PCL) NFs were prepared by electrospinning using a mixture of dimethylformamide (DMF) and dichloromethane (DCM) as the solvent. NFs of different thicknesses were used, with their thickness controlled by varying the electrospinning time of a 12% PCL solution. The resulting NFs were analysed using optical microscopy and scanning electron microscopy. Primary MSCs were seeded onto the prepared NFs, and their osteogenic differentiation capacity was evaluated. Appropriate control samples were also included for comparison. Osteogenic differentiation was assessed by Alizarin Red S staining and quantitative measurement of the absorbance of the extracted dye. We found that NFs promote the osteogenic differentiation of MSCs, with a more pronounced effect observed at greater NF thickness. The results indicate that the osteogenic differentiation of MSCs can be influenced through the appropriate design of PCL NF properties, opening up possibilities for the further development and optimisation of NF scaffolds for use in bone tissue engineering and regenerative medicine. Mezenhimske matične/stromalne celice (MSC) osteogena diferenciacija nanovlakna (NV) elektrostatsko sukanje polikaprolakton (PCL) Mesenchymal stem/stromal cells (MSC) osteogenic differentiation nanofibers (NF) electrospinning polycaprolactone (PCL) true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-09-17 08:46:09 2026-09-17 08:46:16 2026-09-18 04:30:38 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 129292 33426.pdf 33426.pdf 1 0CC177B43EB1DB103418184EBA287918 8db8b627867e0ba8f532cacb56d3104d3e32efe71ddc31bfa2867db08bc248a2 70bd88b4-b263-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247849 Fakulteta za farmacijo 0 0 0