20.500.12556/RUL-187953 Vzpostavitev in karakterizacija primarnih endometriotičnih celičnih kultur Establishment and characterization of primary endometriotic cell cultures Endometrioza je raznolika bolezen, ki se pojavlja v treh glavnih oblikah: peritonealni, ovarijski in globoki endometriozi. Zato so za raziskovanje njenih celičnih in molekularnih značilnosti pomembni modeli, ki ohranjajo lastnosti izvornega tkiva. Namen magistrskega dela je bil vzpostaviti in okarakterizirati primarne stromalne celične kulture iz peritonealnih endometriotičnih lezij ter oceniti njihovo primernost kot model in vitro. Iz 13 vzorcev, pridobljenih od 12 bolnic, smo po mehanski in encimski razgradnji uspešno vzpostavili 11 proliferirajočih primarnih kultur. Na primarnih kulturah smo ocenili proliferacijo, migracijo in senescenco primarnih kultur, izražanje stromalnih in epitelijskih označevalcev, izražanje od estrogenov odvisnih genov ter odziv celic na progesteron (P4), medroksiprogesteron acetat (MPA) in estradiol (E2). Med kulturami posameznih bolnic smo ugotovili izrazito variabilnost v proliferaciji, migraciji insenescenci, izražanju receptorjev za steroidne hormone (ESR1, ESR2, GPER, PGR) in odzivu na P4, MPA in E2. Vse primarne kulture so izražale vimentin, ne pa citokeratina, kar je potrdilo njihov stromalni fenotip. Izražanje ESR1 in PGR je bilo pri vseh analiziranih primarnih kulturah nižje kot v normalnem endometriju, medtem ko sta bila ESR2 in GPER heterogeno izražena. Menstrualna faza ob odvzemu tkiva ni bila statistično značilno povezana s časom podvojitve populacije, migracijo ali izražanjem analiziranih genov. Vzpostavljene primarne stromalne kulture predstavljajo uporaben in vitro model za nadaljnje raziskovanje peritonealne endometrioze in odražajo izrazito heterogenost bolnic. Endometriosis is a heterogeneous disease that occurs in three main forms: peritoneal, ovarian, and deep endometriosis. Therefore, models that retain the characteristics of the tissue of origin are important for investigating its cellular and molecular features. The aim of this master’s thesis was to establish and characterize primary stromal cell cultures from peritoneal endometriotic lesions and to assess their suitability as an in vitro model. From 13 samples obtained from 12 patients, we successfully established 11 proliferating primary cultures following mechanical and enzymatic digestion. In the primary cultures, we assessed proliferation, migration and senescence, the expression of stromal and epithelial markers, the expression of estrogen-responsive genes, and the cellular response to progesterone (P4), medroxyprogesterone acetate (MPA), and estradiol (E2). Marked variability among cultures from individual donors was observed in proliferation, migration, senescence, the expression of steroid hormone receptors (ESR1, ESR2, GPER, and PGR), and the response to P4, MPA, and E2. All primary cultures expressed vimentin but not cytokeratin, confirming their stromal phenotype. The expression of ESR1 and PGR was lower in all analyzed primary cultures than in normal endometrium, whereas ESR2 and GPER showed heterogeneous expression. The menstrual phase at the time of tissue collection was not significantly associated with population doubling time, migration, or the expression of the analyzed genes. The established primary stromal cultures represent a useful in vitro model for further investigation of peritoneal endometriosis and reflect pronounced interindividual heterogeneity. endometrioza peritonealna endometrioza primarne celične kulture primarne endometriotične celične kulture stromalne celice endometriosis peritoneal endometriosis primary cell cultures primary endometriotic cell cultures stromal cells true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-09-17 07:17:01 2026-09-17 07:17:08 2026-09-18 14:13:14 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 289609 291648259 63140.pdf 63140.pdf 1 DD87BF8F5055F77075EBDC7EFF3240AD 171e9ed757dbd128bcf62a3e6980ec1b49cfa65de5aee8ca0ba34a4624462fba ff4325cb-b256-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247801 Biotehniška fakulteta 0 0 0