20.500.12556/RUL-187780 Kinetic properties and pharmacological modulation of high- and low-affinity dopamine transport in striatal astrocytes of adult rats Astrocytes actively participate in neurotransmitter homeostasis by bidirectional communication with neuronal cells, a concept named the tripartite synapse, yet their role in dopamine (DA) homeostasis remains understudied. In the present study, we investigated the kinetic and molecular mechanisms of DA transport in cultured striatal astrocytes of adult rats. Kinetic uptake experiments were performed using radiolabeled [3H]-DA, whereas mRNA expression of the dopamine, norepinephrine, organic cation and plasma membrane monoamine transporters (DAT, NET, OCTs and PMAT) and DA receptors D1 and D2 was determined by qPCR. Additionally, astrocyte cultures were subjected to a 24 h treatment with the DA receptor agonist apomorphine, the DA receptor antagonist haloperidol and the DA precursor L-DOPA. [3H]-DA uptake exhibited temperature, concentration and sodium dependence, with potent inhibition by desipramine, nortriptyline and decynium-22, suggesting the involvement of multiple transporters. qPCR revealed prominent mRNA expression of the NET, the PMAT and OCT1, alongside lower levels of mRNA for OCT2, OCT3 and the DAT. Notably, apomorphine significantly altered NET, PMAT and D1 mRNA expression, while haloperidol and L-DOPA had a modest impact. Our findings demonstrate that striatal astrocytes aid in DA clearance by multiple transporters, which are influenced by dopaminergic drugs. Our study enhances the understanding of regional DA uptake, paving the way for targeted therapeutic interventions in dopaminergic disorders. striatal astrocytes adult rat dopamine dopamine uptake DAT NET PMAT OCT1 striatni astrociti odrasla podgana dopamin absorpcija dopamina transporter NET transporter PMAT transporter OCT1 true false true Angleški jezik Slovenski jezik Članek v reviji 2026-09-14 11:59:21 2026-09-14 11:59:27 2026-09-15 04:27:36 0000-00-00 00:00:00 2024 0 0 14 str. iss. 10, art. 5135 Vol. 25 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615:616-092 1422-0067 10.3390/ijms25105135 197909507 RAZ_Socan_Vesna_2024.pdf RAZ_Socan_Vesna_2024.pdf 1 B5C5DEAD7E84BE338C38BC9ED4DBE698 c890c350f30ce52238ae2291a3f9a5c2ada11abb0bb793515f537b31098d7009 5253de35-b023-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247531 https://www.mdpi.com/1422-0067/25/10/5135 1 f1909058-b022-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247530 Medicinska fakulteta 0 0 0