20.500.12556/RUL-187756 Smoking and diabetes attenuate number of CD34$^{+}$ haematopoietic stem cells in peripheral blood of patients with advanced peripheral artery disease Peripheral artery disease (PAD) is a globally prevalent problem with limited treatment options, leaving up to a fifth of patients remediless. The emergence of new studies on cell therapy in recent years offers a new promising option for their treatment. Our aim was to explore how the number of CD34$^{+}$ hematopoietic cells in the peripheral blood of PAD patients is associated with patients’ functional as well as atherogenic factors. We selected 30 patients with advanced PAD, recorded their performance in a walking test in standard conditions and sampled their blood for further analysis with an emphasis on CD34$^{+}$ cell selection and counting. No correlation of the CD34$^{+}$ cell number was confirmed with any of the observed laboratory parameters. There was an association between the claudication distance and the number of CD34$^{+}$ cells (r = −0.403, p = 0.046). The number of CD34$^{+}$ cells differed between patients with and without type II diabetes (p = 0.071) and between active smokers, past smokers, and non-smokers (p = 0.035; p = 0.068, p = 0.051, respectively), with both smoking and presence of diabetes type II having a negative effect on the number of CD34$^{+}$ cells. Our study demonstrated a dependence of the CD34$^{+}$ cell number on the patient’s characteristics. CD34$^{+}$ cells peripheral artery disease smoking diabetes type II intermittent claudication celice CD34$^{+}$ periferna arterijska bolezen kajenje sladkorna bolezen tip 2 prekinitvena klavdikacija true false true Angleški jezik Slovenski jezik Članek v reviji 2026-09-13 12:12:13 2026-09-15 07:46:04 2026-09-16 10:22:30 0000-00-00 00:00:00 2023 0 0 10 str. iss. 20, art. 15346 Vol. 24 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.1 1422-0067 10.3390/ijms242015346 177470723 RAZ_Sernek_Barbara_2023.pdf RAZ_Sernek_Barbara_2023.pdf 1 09D2E6375368F4BE6E867BDBA0BCBBEC 6279ebcf86f1334b3f34938bea82f410b985e0f39e7c393146bec97201213523 67373b07-b0c9-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247587 https://www.mdpi.com/1422-0067/24/20/15346 1 8fda81f1-af5b-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247498 Medicinska fakulteta 0 0 0