20.500.12556/RUL-187754 Transcriptomic differentiation of phenotypes in chronic rhinosinusitis and its implications for understanding the underlying mechanisms Chronic rhinosinusitis (CRS) is a multifaceted disease with variable clinical courses and outcomes. We aimed to determine CRS-associated nasal-tissue transcriptome in clinically well-characterized and phenotyped individuals, to gain a novel insight into the biological pathways of the disease. RNA-sequencing of tissue samples of patients with CRS with polyps (CRSwNP), without polyps (CRSsNP), and controls were performed. Characterization of differently expressed genes (DEGs) and functional and pathway analysis was undertaken. We identified 782 common CRS-associated nasal-tissue DEGs, while 375 and 328 DEGs were CRSwNP- and CRSsNP-specific, respectively. Common key DEGs were found to be involved in dendritic cell maturation, the neuroinflammation pathway, and the inhibition of the matrix metalloproteinases. Distinct CRSwNP-specific DEGs were involved in NF-kβ canonical pathways, Toll-like receptor signaling, HIF1α regulation, and the Th2 pathway. CRSsNP involved the NFAT pathway and changes in the calcium pathway. Our findings offer new insights into the common and distinct molecular mechanisms underlying CRSwNP and CRSsNP, providing further understanding of the complex pathophysiology of the CRS, with future research directions for novel treatment strategies. sinusitis sequence analysis transcriptome vnetje sinusov analiza zaporedja transkriptom true false true Angleški jezik Slovenski jezik Članek v reviji 2026-09-13 12:11:56 2026-09-15 08:07:48 2026-09-15 08:08:02 0000-00-00 00:00:00 2023 0 0 16 str. iss. 6, art. 5541 Vol. 24 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.21 1422-0067 10.3390/ijms24065541 145698819 RAZ_Urbancic_Jure_2023.pdf RAZ_Urbancic_Jure_2023.pdf 1 8DB0558360858A4422920916B93E01CD 95e10679-b0cb-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247590 https://www.mdpi.com/1422-0067/24/6/5541 1 87a268e3-af5b-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247496 Medicinska fakulteta 0 0 0