20.500.12556/RUL-187752 The co-expression of estrogen receptors ERα, ERβ, and GPER in endometrial cancer Estrogens have important roles in endometrial cancer (EC) and exert biological effects through the classical estrogen receptors (ERs) ERα and ERβ, and the G-protein–coupled ER, GPER. So far, the co-expression of these three types of ERs has not been studied in EC. We investigated ERα, ERβ, GPER mRNA and protein levels, and their intracellular protein distributions in EC tissue and in adjacent control endometrial tissue. Compared to control endometrial tissue, immunoreactivity for ERα in EC tissue was weaker for nuclei with minor, but unchanged, cytoplasmic staining; mRNA and protein levels showed decreased patterns for ERα in EC tissue. For ERβ, across both tissue types, the immunoreactivity was unchanged for nuclei and cytoplasm, although EC tissues again showed lower mRNA and protein levels compared to adjacent control endometrial tissue. The immunoreactivity of GPER as well as mRNA levels of GPER were unchanged across cancer and control endometrial tissues, while protein levels were lower in EC tissue. Statistically significant correlations of estrogen receptor α (ESR1) versus estrogen receptor β (ESR2) and GPER variant 3,4 versus ESR1 and ESR2 was seen at the mRNA level. At the protein level studied with Western blotting, there was significant correlation of ERα versus GPER, and ERβ versus GPER. While in clinical practice the expression of ERα is routinely tested in EC tissue, ERβ and GPER need to be further studied to examine their potential as prognostic markers, provided that specific and validated antibodies are available. endometrial cancer immunohistochemistry GPER rak endometrija imunohistokemija GPER true false true Angleški jezik Slovenski jezik Članek v reviji 2026-09-13 12:11:39 2026-09-16 10:16:29 2026-09-17 04:29:39 0000-00-00 00:00:00 2023 0 0 23 str. iss. 3, art. 3009 Vol. 24 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 618.1 1422-0067 10.3390/ijms24033009 141173251 RAZ_Hojnik_Marko_2023.pdf RAZ_Hojnik_Marko_2023.pdf 1 1F35FEE0939A52EFFFC3F8DBD498FF58 5485f6e80de5c03326661d06fda9bd0482d461e340d19e4137f7729c20769a52 d58c0c42-b0ce-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247591 https://www.mdpi.com/1422-0067/24/3/3009 1 7de5b453-af5b-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=247494 Medicinska fakulteta 0 0 0