20.500.12556/RUL-187354 Razvoj in validacija LC-MS metode za določanje rastlinskih sterolov v bioloških vzorcih Development and validation of an LC-MS method for the determination of plant sterols in biological samples Fitosteroli so steroli rastlinskega izvora, ki v rastlinah opravljajo vlogo, ki jo opravlja holesterol v živalskih celicah. Človeško telo jih ne more sintetizirati, zato jih zaužijemo z rastlinsko hrano, predvsem z zelenjavo in rastlinskimi olji. Povezani so z mnogimi pozitivnimi učinki, od zniževanja ravni holesterola v krvi do protirakavih učinkov, povezani pa so tudi z boleznimi. Najpogosteje jih v bioloških vzorcih določamo s plinsko kromatografijo, sklopljeno z masno spektrometrijo, v tem diplomskem delu pa smo razvili metodo tekočinske kromatografije, sklopljene s tandemsko masno spektrometrijo. Optimizirali in validirali smo metodo v širokem linearnem območju z visoko ponovljivostjo. S poskusom optimizacije protokola za izolacijo sterolov iz človeške plazme smo potrdili, da je bil prvotni protokol že optimiziran. Phytosterols are plant-derived sterols that perform a role in plants similar to that of cholesterol in animal cells. The human body is unable to synthesize them, so they are obtained through a plant-based diet, manly from vegetables and vegetable oils. They are associated with numerous beneficial effects, ranging from lowering blood cholesterol levels to anticancer effects, but they are also linked to certain diseases. In biological samples, they are most commonly determined using gas chromatography, coupled with mass spectrometry, however, in this thesis, we developed a method based on liquid chromatography coupled with tandem mass spectrometry. The method was optimised and validated over a broad linear range and showed high repeatability. By attempting to optimize the protocol for sterol isolation from human plasma, we confirmed that the existing protocol was already optimized. fitosteroli sitosterol kampesterol stigmasterol ergosterol LC-MS phytosterols sitosterol campesterol stigmasterol ergosterol LC-MS true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-09-10 11:10:03 2026-09-10 11:10:09 2026-10-28 10:44:20 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 26163 302848003 Matek_Nik_-_Razvoj_in_validacija_LC-MS_metode_za_dolocanje_rastlinskih_sterolov_v_bioloskih_vzor.pdf Matek_Nik_-_Razvoj_in_validacija_LC-MS_metode_za_dolocanje_rastlinskih_sterolov_v_bioloskih_vzor.pdf 1 E8F21AB640A1C32468A19206D48D0BC7 a53a79b4808f67277f222ecebf02ecb4b1abaf10ac9799c311478106b9ef5972 674e5c76-acf7-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=246968 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0