20.500.12556/RUL-187280 Termodinamska stabilnost nanotelesa SG4 Thermodynamic Stability of Nanobody SG4 Namen tega diplomskega dela je bil eksperimentalno preučiti termodinamsko stabilnost in konformacijske spremembe nanotelesa SG4. Pri raziskavi smo uporabili in primerjali rezultate dveh komplementarnih fizikalno-kemijskih metod: diferenčne dinamične kalorimetrije (DSC) in UV-Vis spektroskopije. Eksperimenti z diferenčno dinamično kalorimetrijo so omogočili neposredno spremljanje toplotne denaturacije nanotelesa SG4 ter določitev ključnih termodinamskih parametrov, kot so temperatura polovice prehoda, kalorimetrična entalpija in van't Hoffova entalpija. Izvedba več zaporednih ciklov segrevanja in ohlajanja je služila kot metoda za preverjanje reverzibilnosti konformacijskega prehoda SG4. UV-Vis spektroskopska analiza je bila uporabljena kot dodatna neodvisna metoda za preučevanje odvisnosti absorbance od temperature. Rezultati obeh metod za nanotelo SG4 so bili medsebojno primerjani z vidika predpostavk modela dveh stanj in fizikalne osnove samih meritev. Pridobljena spoznanja prispevajo k boljšemu razumevanju stabilnosti struktur nanoteles. The aim of this diploma thesis was to experimentally investigate the thermodynamic stability and conformational changes of the SG4 nanobody. In this study, we employed and compared the results of two complementary physicochemical methods: differential scanning calorimetry (DSC) and UV-Vis spectroscopy. Differential scanning calorimetry experiments enabled direct monitoring of the thermal denaturation of the SG4 nanobody and the determination of key thermodynamic parameters, such as the midpoint transition temperature, calorimetric enthalpy, and van 't Hoff enthalpy. Conducting multiple consecutive heating and cooling cycles served as a method to evaluate the reversibility of the conformational transition of SG4. UV-Vis spectroscopic analysis was used as an additional independent method to study the temperature dependence of absorbance. The results obtained for the SG4 nanobody using both methods were compared regarding the assumptions of the two-state model and the physical background of the measurements themselves. The insights gained contribute to a better understanding of nanobody structural stability. Ključne besede: G-kvadrupleks nanotelo SG4 i-motiv diferenčna dinamična kalorimetrija (DSC) UV-Vis spektroskopija termodinamska stabilnost reverzibilnost Keywords: G-quadruplex nanobody SG4 i-motif differential scanning calorimetry (DSC) UV-Vis spectroscopy thermodynamic stability reversibility. true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-09-10 06:55:03 2026-09-10 06:55:16 2026-09-11 04:28:08 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 26099 Lutvica_Lejla_-_Termodinamska_stabilnost_nanotelesa_SG4.pdf Lutvica_Lejla_-_Termodinamska_stabilnost_nanotelesa_SG4.pdf 1 05466F61751628B50893D566CB3229CC f8a2dd0ea3cd421d7bc6dbb8c56fd1ffcacc0122b45b77dbfb8add8f1a842628 cb913758-acd3-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=246830 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0