20.500.12556/RUL-187225 De novo načrtovanje proteinskega vezalca na mikrotubule De novo designing of a protein binder against microtubules Proteini, ki se specifično vežejo na tarčno molekulo, so hitro pritegnili pozornost znanstvenikov in farmacevtske industrije. Z njimi lahko označujemo posamezne strukture v raziskavah, ob izbiri prave tarče pa lahko vplivajo na potek bolezni. Ostajalo pa je vprašanje, kako de novo načrtovati vezalce na želeno tarčo. Z razvojem umetne inteligence je tudi ta cilj postal dosegljiv. Za tarčo smo si izbrali mikrotubule in s tremi različnimi programi načrtovali 27 potencialnih vezalcev. Izrazili smo jih s fluorescenčnim fuzijskim partnerjem in očistili, nato pa analizirali s fluorescenčno mikroskopijo s popolnim notranjim odbojem (TIRF). V tej magistrski nalogi smo pokazali, da je s trenutno dostopnimi orodji umetne inteligence možno načrtovati proteine, ki se vežejo na mikrotubule. Proteins which specifically bind to a target molecule have quickly gained the interest of researchers and the pharmaceutical industry alike. They can be used as markers for different cell structures or molecules, and if the target is chosen correctly, they can affect the progress of a disease. But an important gap remained: how to de novo design binders towards a chosen target? Rapid advances in artificial intelligence tailored to this task made this goal reachable. We chose microtubules as the target and used three different programs to design 27 potential binder candidates. We expressed them fused with a fluorescent protein. After isolation and chromatography, the binding was analysed using total internal reflection fluorescence (TIRF) microscopy. In this research, we show that currently available AI driven tools allow us to design novel proteins binding to a chosen target. vezalec mikrotubul Prosculpt BindCraft BoltzGen binder microtubule Prosculpt BindCraft BoltzGen true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-09-09 12:05:17 2026-09-09 12:05:20 2026-09-11 03:35:36 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 25722 Znidar_Zan_-_De_novo_nacrtovanje_proteinskega_vezalca_na_mikrotubule.pdf Znidar_Zan_-_De_novo_nacrtovanje_proteinskega_vezalca_na_mikrotubule.pdf 1 8B19D9139C2989359F3EBB29740A3283 62cd423ebd0ab8d0663c193c06c6beec9ab688e1b3f24693021f0c43f72e3064 f21f0adf-ac35-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=246731 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0