20.500.12556/RUL-186890 Optimizacija in vitro rasti korenin Nicotiana benthamiana in antagonizem med glivama Trichoderma harzianum ter Rhizoctonia solani Optimization of In Vitro Root Growth of Nicotiana benthamiana and Antagonism of Trichoderma harzianum against Rhizoctonia solani V diplomskem delu smo preučevali vpliv različnih hormonskih gojišč na razvoj koreninskega sistema modelne rastline Nicotiana benthamiana ter antagonistične interakcije med izbranimi glivnimi sevi. Rastline smo gojili na gojiščih z različnimi koncentracijami rastnih regulatorjev IBA, BAP in NAA ter na kontrolnem gojišču brez dodanih rastnih regulatorjev. Spremljali smo razvoj korenin, tvorbo kalusa in poganjkov ter rast rastlin. Najbolj razvit koreninski sistem so razvile rastline na kontrolnem gojišču, medtem ko je pri večini hormonskih gojišč prevladovala tvorba kalusa ali poganjkov. V drugem delu raziskave smo na PDA-gojišču spremljali medsebojno rast šestih glivnih sevov iz rodov Fusarium in Trichoderma. Antagonistične interakcije smo vrednotili na podlagi nastanka inhibicijskih oziroma demarkacijskih con in odsotnosti medsebojnega preraščanja kolonij. Opazili smo jih le pri nekaterih kombinacijah, predvsem med sevi rodu Trichoderma. Rezultati kažejo, da uporabljeni rastni regulatorji niso izboljšali razvoja korenin v primerjavi s kontrolnim gojiščem ter da so bile antagonistične interakcije odvisne od posamezne kombinacije glivnih sevov. This thesis examined the effects of different hormone supplemented media on root system development in the model plant Nicotiana benthamiana, as well as antagonistic interactions among selected fungal strains. Plants were grown on media containing different concentrations of the plant growth regulators IBA, BAP and NAA, and on a control medium without added growth regulators. Root development, callus and shoot formation, and overall plant growth were monitored. The most developed root system was observed in plants grown on the control medium, while callus or shoot formation predominated on most hormone-supplemented media. In the second part of the study, interactions among six fungal strains from the genera Fusarium and Trichoderma were monitored on PDA medium. Antagonistic interactions were evaluated based on the formation of inhibition zones and the absence of colony overgrowth. Such interactions were observed only in some combinations, mainly between strains of the genus Trichoderma. The results show that the tested plant growth regulators did not improve root development compared with the control medium and that antagonistic interactions depended on the specific combination of fungal strains. Nicotiana benthamiana rast korenin rastni regulatorji Trichoderma harzianum antagonizem biološko varstvo Nicotiana benthamiana root growth plant growth regulators Trichoderma harzianum antagonism biological control true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-09-06 08:16:21 2026-09-06 08:16:24 2026-09-08 14:32:53 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 287184 290411011 61202.pdf 61202.pdf 1 C0E17BA147C7F9355A2F22634084113D ced897982a0c87c3e5f47c157dad385e2096285a9130aaa982e7800dff4b10c9 79b0d483-a9ba-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=246272 Biotehniška fakulteta 0 0 0