20.500.12556/RUL-186750 The influence of evaluator experience, dynamic versus static ultrasound image interpretation, and probe frequency on fascicle differentiation Purpose: To determine the influence of evaluator experience, dynamic versus static ultrasound (US) image interpretation, and probe frequency on fascicle differentiation. Methods: Evaluator experience and dynamic versus static US image interpretation were assessed on 12 cadaveric forearms. Median nerves were scanned using a 23-MHz US probe, followed by nerve excision and high-field magnetic resonance imaging (MRI) at 9.4-T. Five independent evaluators with different experience levels delineated fascicles twice, using static and dynamic US images. Delineations were compared with high-field MRI. The influence of probe frequency was evaluated on 12 median nerves of healthy volunteers. Median nerves at wrist were scanned using 14-, 18-, 24-, and 70-MHz US probes. Images were cross-referenced, and fascicle counts were compared relative to the highest-frequency probe. Results: Dynamic imaging improved fascicle differentiation by 27.6% to 51.2% compared to static (P < .001), with a greater benefit for less experienced evaluators. The most experienced evaluator identified on average 62.3% more fascicles in dynamic assessment compared to the least experienced. The 70-MHz probe showed the highest mean fascicle count (27.6 ± 3.7), while 24-, 18-, and 14-MHz probes differentiated 26%, 46%, and 63% fewer fascicles, respectively (P <. 0001). Conclusions: Fascicle differentiation improves significantly with evaluator experience, using dynamic US image interpretation, and higher probe frequency. These findings support the clinical utility of ultrahigh-frequency US for detailed evaluation of superficial nerves. Clinical relevance: Experience and dynamic image interpretation are important for reliable fascicle recognition and to minimize misinterpretation. Ultrahigh-frequency US probes substantially improve fascicle differentiation, but their limited penetration requires careful selection of probe frequency based on nerve depth. differentiation experience fascicle image analysis magnetic resonance microscopy probe ultrasonography diferenciacija izkušnja živčni snopič mišični snopič slikovna analiza magnetnoresonančna mirkoskopija sonda ultrasonografija true false true Angleški jezik Slovenski jezik Članek v reviji 2026-09-04 13:31:59 2026-09-04 13:32:07 2026-09-05 04:34:26 0000-00-00 00:00:00 2026 0 0 9 str. iss. 9 Vol. 51 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-07 1531-6564 10.1016/j.jhsa.2026.04.004 281469443 RAZ_Pusnik_Luka_2026.pdf RAZ_Pusnik_Luka_2026.pdf 1 B09BDE29B04AB2397E6F4B4FF0FE1D7A ed987781d6a6ff65461e55750399dbbff41b925d73b5d6ff4e8ac37e810db4a0 da767e4e-a854-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=246116 https://www.jhandsurg.org/article/S0363-5023(26)00309-6/fulltext 1 433a232c-a854-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=246115 Medicinska fakulteta 0 0 0