20.500.12556/RUL-186660 Karakterizacija genomov amdoparvovirusov mačjih pand ter analiza viroma v fekalnih vzorcih mačjih pand iz evropskih živalskih vrtov Genome analysis of red panda amdoparvoviruses and virome analysis in fecal samples from red pandas from European zoos Analizirali smo 27 vzorcev fecesa mačjih pand in dva vzorca tkiv poginulih mačjih pand iz evropskih živalskih vrtov, pri katerih smo s PCR ugotovili prisotnost RpAPV. Z uporabo NGS ter sekvenciranja po Sangerju smo uspešno sestavili pet skoraj celih genomov ter 19 odsekov genov NS in VP RpAPV. Z uporabo programa MEGA12 smo opravili filogenetsko analizo, ki je pokazala, da vsi sevi pripadajo vrsti Amdoparvovirus carnivoran 5, oblikujejo pa svojo filogenetsko linijo znotraj te vrste. Virusa nam ni uspelo izolirati na celičnih linijah. Z uporabo NGS smo določili prisotnost ter zastopanost virusnih družin v fecesu mačjih pand. Rezultati kažejo na dominantno prisotnost štirih fagnih družin (Herelleviridae, Myoviridae, Podoviridae, Siphoviridae) ter štirih družin sesalskih virusov (Circoviridae, Picobirnaviridae, Orthomyxoviridae, Parvoviridae), kar se sklada z rezultati prejšnjih raziskav fekalnega viroma in mikrobioma mačjih pand. We analyzed 27 fecal samples from red pandas and two mixed tissue samples from deceased red pandas from European zoos that tested positive for RpAPV by PCR. Using NGS and Sanger sequencing, we successfully assembled five complete and 19 partial RpAPV genomes. Using the MEGA12 software, we performed a phylogenetic analysis, which determined that each genome belongs to the species Amdoparvovirus carnivoran 5, yet together they form a distinct phylogenetic lineage within this species. We were unable to isolate the virus on cell lines. Using NGS, we determined the presence and abundance of viral families in red panda feces. The results indicate the dominant presence of four phage families (Herelleriviridae, Myoviridae, Podoviridae, Siphoviridae) and four families of mammalian viruses (Circoviridae, Picobirnaviridae, Orthomyxoviridae, Parvoviridae), which is consistent with the results of previous studies of the red panda fecal virome and microbiome. mačji panda Ailurus fulgens amdoparvovirus RpAPV NGS fekalni virom red panda Ailurus fulgens amdoparvovirus RpAPV NGS fecal virome true false false [M. Perović] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-09-04 07:17:25 2026-09-04 07:17:30 2026-09-05 04:33:49 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 578.5:599.74 286859 290021123 60534.pdf 60534.pdf 1 D9D6CA47E78B2B98379F361775307506 d2d1895fa61281179017a30c178aeb4e5ba57be0b0f30141a49a7946857f5fb0 ed78b70c-a81f-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245977 Biotehniška fakulteta 0 0 0