20.500.12556/RUL-186600 Izražanje receptorjev za inzulin in inzulinu podobni rastni dejavnik 1 v kulturi skeletnomišičnih celic Expression of insulin receptors and insulin-like growth factor 1 in skeletal muscle cell culture Skeletna mišica predstavlja pomembno tarčno tkivo za delovanje inzulina in ima ključno vlogo pri uravnavanju presnove glukoze ter vzdrževanju energijskega ravnovesja organizma. Učinki inzulina in inzulinu podobnega rastnega dejavnika 1 (IGF-1) so posredovani preko inzulinskega receptorja (IR) ter receptorja za inzulinu podobni rastni dejavnik 1 (IGF-1R), ki zaradi velike strukturne homologije aktivirata številne skupne signalne poti, hkrati pa opravljata različni fiziološki vlogi. Poznavanje njunega izražanja v različnih modelih skeletne mišice je pomembno za razumevanje signalizacije, ki uravnava presnovo, rast in regeneracijo mišičnega tkiva. Namen diplomskega dela je bil določiti relativno izražanje genov INSR in IGF1R v diferenciranih primarnih človeških skeletnomišičnih celicah ter genov Insr in Igf1r v diferencirani podganji celični liniji L6. Iz celic smo izolirali skupno RNA, sintetizirali cDNA in relativno izražanje določili z metodo kvantitativne verižne reakcije s polimerazo. Dobljeni rezultati so pokazali, da sta bila oba gena izražena v obeh celičnih modelih. Relativni ravni mRNA obeh genov sta bili statistično višji v celični liniji L6 kot v primarnih človeških skeletnomišičnih celicah. V celični liniji L6 je bila relativna raven mRNA Igf1r statistično značilno višja od Insr, medtem ko se relativni ravni mRNA IGF1R in INSR v primarnih človeških skeletnomišičnih celicah nista statistično značilno razlikovali. Primerljiv vzorec rezultatov smo dobili pri vseh treh načinih normalizacije, pri čemer sta bila kot referenčna gena uporabljena PPIA in 18S rRNA. Raziskava prispeva k boljšemu razumevanju izražanja receptorjev za inzulin in IGF-1 v dveh pogosto uporabljenih in vitro modelih skeletne mišice ter predstavlja izhodišče za nadaljnje raziskave njunega izražanja in funkcionalne vloge v skeletni mišici. Skeletal muscle is a major target tissue for insulin action and plays a central role in regulating glucose metabolism and maintaining whole-body energy homeostasis. The biological effects of insulin and insulin-like growth factor 1 (IGF-1) are mediated through the insulin receptor (IR) and the insulin-like growth factor 1 receptor (IGF-1R). Owing to their high structural homology, these receptors activate numerous common intracellular signaling pathways while performing distinct physiological functions. Understanding their expression in different skeletal muscle models is essential for elucidating the signaling mechanisms involved in the regulation of muscle metabolism, growth, and regeneration. The aim of this bachelor's thesis was to determine the relative expression of the INSR and IGF1R genes in differentiated primary human skeletal muscle cells and the Insr and Igf1r genes in differentiated rat L6 skeletal muscle cells. Total RNA was isolated from the cells, cDNA was synthesized, and relative gene expression was determined using quantitative polymerase chain reaction. The results showed that both genes were expressed in both cell models. The relative mRNA levels of both genes were statistically significantly higher in the L6 cell line than in primary human skeletal muscle cells. In the L6 cell line, the relative Igf1r mRNA level was statistically significantly higher than that of Insr, whereas the relative mRNA levels of IGF1R and INSR did not differ statistically significantly in primary human skeletal muscle cells. A comparable pattern of results was obtained with all three normalization approaches, using PPIA and 18S rRNA as reference genes. This study contributes to a better understanding of the expression of insulin and IGF-1 receptors in two widely used in vitro models of skeletal muscle and provides a basis for future investigations of their expression and functional role in skeletal muscle. skeletnomišične celice inzulinski receptor receptor za inzulinu podobni rastni dejavnik 1 skeletal muscle cells insulin receptor insulin-like growth factor 1 receptor true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-09-03 09:45:00 2026-09-03 09:45:14 2026-09-29 11:05:13 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 26201 292876547 Mulalic_Anita_-_Izrazanje_receptorjev_za_inzulin_in_inzulinu_podobni_rastni_dejavnik_1_v_kulturi.pdf Mulalic_Anita_-_Izrazanje_receptorjev_za_inzulin_in_inzulinu_podobni_rastni_dejavnik_1_v_kulturi.pdf 1 DFD37EC8ABABB93AB1AE6854F01F219C bb23ac8edd2559768da5935f6363ea87a49f804ea7923d34226d6a0dbde82bfb 66a110d9-a76b-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245878 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0