20.500.12556/RUL-186531 Expression of miR-155, miR-223, miR-29b, miR-142-3p, and miR-142-5p in kidney allografts with acute tubular necrosis, rejection phenotypes, and BK-polyomavirus nephropathy Specific tissue microRNA (miRNA) profiles may reflect biologically relevant pathways of kidney allograft injury and rejection. We analyzed the intragraft expression of five preselected miRNAs (miR-29b-3p, miR-142-3p, miR-142-5p, miR-155-5p and miR-223-3p) using qPCR in biopsies from patients with antibody-mediated rejection (ABMR; n = 26), T cell-mediated rejection (TCMR; n = 14), BK polyomavirus nephropathy (BKVN; n = 15), acute tubular necrosis (ATN; n = 8), and controls (CTRL; n = 20). miR-155-5p, miR-223-3p, and miR-142-5p/3p were significantly up-regulated in ABMR (p < 0.01), TCMR (p ≤ 0.012), and BKVN (p ≤ 0.001) compared with CTRL, while miR-223-3p levels in ATN were comparable to CTRL. These immune-related miRNAs correlated with serum creatinine, proteinuria, Banff inflammation scores (t, i, ti; all p ≤ 0.001), and transcriptomic markers of rejection and tissue injury derived from molecular analysis of biopsy samples (all p < 0.01). miR-155-5p showed the strongest and most consistent association with rejection-related parameters, whereas miR-29b-3p correlated with donor characteristics and chronic fibrosis. In classification analyses, individual miRNAs displayed moderate discrimination, with miR-223-3p performing best, while a combined multi-miRNA model markedly improved separation of rejecting and non-rejecting phenotypes (AUC = 0.954 after BKVN exclusion). Intragraft miRNA expression was associated with selected inflammatory and immune-mediated features of allograft dysfunction and may provide supportive biological information alongside histopathological and molecular assessment in future studies. However, these exploratory findings require validation in larger, independent cohorts, including paired blood or urine samples, before any diagnostic or biomarker role can be established. miRNA Kidney transplantation Antibody-mediated rejection Acute tubular necrosis BK polyomavirus nephropathy T cell-mediated rejection mikroRNA presatidev ledvic protitelesno posredovana zavrnitev akutna tabularna nekroza BK-virusna nefropatija s celicami T posredovana zavrnitev true false true Angleški jezik Slovenski jezik Članek v reviji 2026-09-03 04:36:11 2026-09-24 09:46:55 2026-09-25 04:27:50 0000-00-00 00:00:00 2026 0 0 27 str. art. 28401 Vol. 16 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.61-089.843 2045-2322 10.1038/s41598-026-59410-0 284000259 RAZ_Bezeljak_Neva_2026.pdf RAZ_Bezeljak_Neva_2026.pdf 1 719BF018BD963CFD09672DBA1DDE9CB8 02e618acdcfe0762883277785e281d5b33b5a3f64055562772e0193443a8ec5b 6c395f68-b7f0-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=248866 https://www.nature.com/articles/s41598-026-59410-0 1 d22effb6-a73f-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245804 Medicinska fakulteta 0 0 0