20.500.12556/RUL-186483 Uporaba modificirane elektrode za elektrokemijsko določitev antibiotika sulfametoksazola v vodnem mediju Use of a modified electrode for the electrochemical determination of the antibiotic sulfamethoxazole in an aqueous medium Sulfametoksazol (SMX) je eden izmed pogosto uporabljenih antibiotikov, čigar koncentracija v okolju narašča, kar vzbuja skrb zaradi razvoja bakterijske odpornosti, hkrati pa ima konstantna izpostavljenost antibiotikom v vodi negativne učinke na človeško zdravje. Med klasične analizne metode za določitev antibiotikov v vodnem mediju spadajo predvsem različice kromatografskih tehnik, vendar se razvoj v zadnjem času usmerja k hitrejšim, cenovno ugodnejšim in prenosnim načinom določitve, kjer prevladujejo elektrokemijski senzorji. V magistrskem delu je bil izdelan elektrokemijski senzor za določitev SMX na osnovi sitotiskane elektrode (SPE) z ogljikovo delovno elektrodo, ki je bila modificirana s prevodnim polimerom in dodatnim ogljikovim materialom. V prvem delu je predstavljena primerjava med dvema tipoma ogljikovih elektrod: SPE z ogljikovo delovno elektrodo (SPE-C) in SPE z delovno elektrodo iz enostenskih ogljikovih nanocevk (SPE-SWCNT). Na obeh elektrodah so bile preizkušene modifikacije s prevodnimi polimeri, ogljikovimi nanomateriali in nanomateriali kovinskih oksidov. Najboljši odziv je pokazala modifikacija s kombinacijo polimetilen modrega in ogljikovih kvantnih pik. Modificirana elektroda je bila v drugem delu uporabljena za določitev elektrokemijskih lastnosti sistema in umeritvene premice, s pomočjo katere sta bili določeni meja določitve (0,1 μM) in meja zaznave (0,06 μM). V tretjem delu je elektrokemijska določitev primerjana z določitvijo s tekočinsko kromatografijo visoke ločljivosti (HPLC), kjer se je izkazalo, da sta tehniki za določitev nizkih koncentracij (okoli 0,1 μM) SMX primerljivi. Sulfamethoxazole (SMX) is one of the most used antibiotics, and its concentration in the environment is increasing, which is raising concerns about the development of bacterial resistance. At the same time, the continuous presence of antibiotics in water results in adverse effects on human health. Conventional analytical methods for the determination of antibiotics in aqueous media are mainly based on chromatographic techniques. However, recent developments have focused on faster, more cost-effective, and portable analytical approaches, among which the electrochemical sensors have become increasingly prominent. In present master's thesis, an electrochemical sensor for the determination of SMX was developed based on a screen-printed electrode (SPE) with a carbon working electrode modified with a conductive polymer and an additional carbon material. The first part presents a comparison between two types of carbon-based electrodes: a screen-printed electrode with a carbon working electrode (SPE-C) and a screen-printed electrode with a single-walled carbon nanotube working electrode (SPE-SWCNT). Both electrodes were modified using conductive polymers, carbon nanomaterials, and metal oxide nanomaterials. The modification based on a combination of polymethylene blue and carbon quantum dots exhibited the best response. In the second part, the modified electrode was used to investigate the electrochemical properties of the system and to construct a calibration curve, from which the limit of quantification (0.1 μM) and the limit of detection (0.06 μM) were determined. In the third part, the electrochemical determination is compared with high-performance liquid chromatography (HPLC), demonstrating that both techniques provide comparable results for the determination of low SMX concentrations (down to 0.1 μM). sulfametoksazol voltametrija sitotiskane elektrode modifikacija ogljikovi nanomateriali sulfamethoxazole voltammetry screen-printed electrodes modification carbon nanomaterials true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-09-02 11:06:46 2026-09-02 11:06:59 2026-09-24 12:00:36 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 25997 292357635 Hadolin_Melanija_-_Uporaba_modificirane_elektrode_za_elektrokemijsko_dolocitev_antibiotika_sulfa.pdf Hadolin_Melanija_-_Uporaba_modificirane_elektrode_za_elektrokemijsko_dolocitev_antibiotika_sulfa.pdf 1 E73A674AF3559F65C31C41B3ADE624FA a539d10b0b7342f38d68320ec8840c45dc9c773beabcbb5ba7c0b4f022b1fa9b 3b9c67e7-a6ad-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245746 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0