20.500.12556/RUL-186390 Priprava polimernih struktur iz aktiviranih karboranskih reagentov in peptidov Polymer preparation from reactive carborane reagents and peptides Dikarba-closo-dodekaborani so termično in kemijsko stabilni ter imajo enkratno tridimenzionalno strukturo. Karborani so zaradi svoje visoke vsebnosti bora zanimivi predvsem za BNCT (angl. Boron Neutron Capture Therapy). Njihova vgradnja v polipeptidne verige omogoča pripravo tarčnih in učinkovitih dostavnih sistemov bora v tumorske celice. V okviru diplomskega dela smo sintetizirali homo-bifunkcionalni karboranski reagent z dvema N-hidroksisukcinimidnima (NHS) estrskima skupinama, ki je služil kot povezovalni člen med peptidnimi verigami. Z avtomatizirano metodo Fmoc-SPPS (angl. Solid-Phase Peptide Synthesis) smo pripravili peptide različnih aminokislinskih zaporedij in dolžin. Polimerne strukture smo sintetizirali pod različnimi pogoji in tako preizkusili, kako različne sekvence peptidov, razmerje med reagentom in peptidom ter reakcijska temperatura vplivajo na dolžino nastalih polimerov. Prisotnost histidina v bližini lizina se je izkazala za ključno pri tvorbi stabilne amidne vezi med karboranskim reagentom in peptidom. Za tvorbo daljših polimerov brez neželenega vlečenja lis na SDS-PAGE gelu je bilo optimalno razmerje 1:4 (reagent : peptid) pri 50 °C. Dicarba-closo-dodecaboranes possess thermal and chemical stability as well as a unique three-dimensional structure. Due to their high boron content, carboranes are of particular interest for BNCT (Boron Neutron Capture Therapy). Their incorporation into polypeptide chains enables the preparation of targeted and effective boron delivery systems to tumor cells. As part of this thesis, we synthesized a homo-bifunctional carborane reagent with two N-hydroxysuccinimide (NHS) ester groups, which served as a linking moiety between peptide chains. Using automated method Fmoc-SPPS (Solid-Phase Peptide Synthesis), we prepared peptides with different amino acid sequences and lengths. We synthesized the polymer structures under various conditions to investigate how different peptide sequences, the reagent-to-peptide ratio, and reaction temperature affect the length of the resulting polymers. The presence of histidine in the vicinity of lysine proved to be crucial for the formation of a stable amide bond between the carborane reagent and the peptide. To form longer polymers without unwanted smearing on the SDS-PAGE gel, the optimal ratio was 1:4 (reagent : peptide) at 50 °C. BNCT karborani peptidi polimeri NHS BNCT carboranes peptides polymers NHS true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-09-01 07:45:20 2026-09-01 07:45:33 2026-09-03 04:37:43 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2027-08-31 25984 Logar_Petja_-_Priprava_polimernih_struktur_iz_aktiviranih_karboranskih_reagentov_in_peptidov.pdf Logar_Petja_-_Priprava_polimernih_struktur_iz_aktiviranih_karboranskih_reagentov_in_peptidov.pdf 1 10560C9C80DE548997865D356825D260 f5e2c85668586b39a09d5d0de8c3d339c26542ecd000bf9721b15d16e1607bcf eed8c353-a5c7-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245628 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0