20.500.12556/RUL-186378 The illusion of sharp boundaries unresolved issues in reversible and irreversible electroporation Electroporation has birthed two massive parallel universes of applications: medicine and food processing. Despite operating on the same fundamental physics, these fields fail to crosspollinate, limiting their advancement. The classical “aqueous pore” model and threshold-based definitions fail to explain the highly complex, multiparameter realities of molecular transport, cell death mechanisms, and tissue-level physiological responses. The fields often struggle to translate in vitro findings—where even varying cell lines yield conflicting results—into predictive clinical and industrial outcomes. Upscaling from small animals to humans, as well as from laboratory to industrial scale, often fails due to increasing biological complexity and variability. This translational chasm is exacerbated by a lack of multiscale and multiphysics modeling that couples electric fields with thermodynamics, fluid dynamics, and electrochemistry and most of all downstream effects of electroporation like cell death and changes in mass transport. Emerging clinical applications like Pulsed Field Ablation and therapies based on gene delivery represents a perplexing, multiparameter optimization problem that must balance electrical, thermal, and electrochemical constraints. This paper highlights critical deficiencies, explores the sharp boundary between reversible and irreversible electroporation, and calls for better reporting, standardized nomenclature, and interdisciplinary collaboration to elevate electroporation from an empirical to a universally predictive, robust science. irreversible electroporation drug delivery cardiac ablation gene delivery food processing multiphysics modeling pulsed field ablation reverzibilna elektroporacija ireverzibilna elektroporacija vnos zdravil srčna ablacija vnos genov predelava hrane multifizikalno modeliranje ablacija s pulznim električnim poljem true false true Angleški jezik Slovenski jezik Članek v reviji 2026-08-31 13:28:09 2026-08-31 13:28:19 2026-09-03 04:37:38 0000-00-00 00:00:00 2027 0 0 10 str. art.109416 Vol. 173 Feb. 2027 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 602.621:621.31 1567-5394 10.1016/j.bioelechem.2026.109416 287253251 1-s2.0-S1567539426002021-main.pdf 1-s2.0-S1567539426002021-main.pdf 1 1FBBB74242B4CA759427C7F02AD80F93 b1cf2e7ff433ee41453fdcf2d1ff5f5de14fd7615c9f08e9ff0be2240f9327c5 dab04c7e-a52e-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245590 https://www.sciencedirect.com/science/article/pii/S1567539426002021 1 a6072059-a52e-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245588 Fakulteta za elektrotehniko 0 0 0