20.500.12556/RUL-186281 Vezava kurkumina in njegovih analogov na G-kvadruplekse The Binding of Curcumin and Its Analogues to G-Quadruplexes G-kvadrupleksi (G4) so nekonvencionalne strukture DNA, ki se pojavljajo v z gvaninom bogatih zaporedjih in imajo pomembno vlogo pri uravnavanju izražanja genov. Zaradi prisotnosti v promotorskih regijah onkogenov predstavljajo potencialne molekularne tarče za razvoj novih protitumorskih učinkovin. Kurkumin je naravni polifenol, ki se lahko veže na različne topologije G4, njegovi analogi pa omogočajo raziskovanje vpliva strukturnih sprememb na afiniteto, način vezave in stabilizacijo teh struktur. Diplomsko delo temelji na pregledu in primerjalni analizi raziskav, ki proučujejo interakcije kurkumina in njegovih analogov z G4. Rezultati kažejo, da se kurkumin večinoma veže z zunanjimi načini vezave, pri čemer na afiniteto in stabilizacijo pomembno vplivata topologija G4 ter kemijska zgradba liganda. Med preučevanimi spojinami je analog Sb-NBC pokazal izrazitejši stabilizacijski učinek in večjo selektivnost do nekaterih tumorskih G4 kot kurkumin. Ugotovitve kažejo, da je z ustreznimi strukturnimi spremembami analogov mogoče izboljšati njihove lastnosti in razviti boljše analoge. Kurkuminovi derivati tako predstavljajo obetavno izhodišče za razvoj novih tarčno usmerjenih protitumorskih učinkovin, vendar ostajajo izzivi njihove selektivnosti, biorazpoložljivosti ter potrditev učinkovitosti in varnosti v kompleksnejših bioloških modelih. G-quadruplexes (G4) are unconventional DNA structures that occur in guanine-rich sequences and play a significant role in the regulation of gene expression. Due to their presence in promoter regions of oncogenes, they represent potential molecular targets for the development of new antitumor agents. Curcumin is a natural polyphenol that can bind to different G4 topologies, while its analogues enable the investigation of the influence of structural modifications on the affinity, binding mode, and stabilization of these structures. The thesis is based on a review and comparative analysis of studies investigating the interactions of curcumin and its analogues with G4. The results show that curcumin mostly binds through external binding modes, with G4 topology and the chemical structure of the ligand significantly influencing affinity and stabilization. Among the compounds studied, the Sb-NBC analogue showed a more pronounced stabilizing effect and greater selectivity towards some tumour-associated G4 than curcumin. The findings indicate that appropriate structural modifications of analogues can improve their properties and enable the development of better analogues. Curcumin derivatives thus represent a promising starting point for the development of new targeted antitumor agents; however, challenges regarding their selectivity and bioavailability, as well as the confirmation of their efficacy and safety in more complex biological models, remain. G-kvadrupleks kurkumin analogi kurkumina protirakava učinkovina vezava ligandov stabilizacija G-kvadrupleksov G-quadruplex curcumin curcumin analogues anticancer agent ligand binding stabilisation of G-quadruplexes true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-08-30 07:18:46 2026-08-30 07:18:52 2026-09-01 10:40:50 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 286311 289570307 60682.pdf 60682.pdf 1 63B4285B98DE3F09CAB6917245207529 36afd0f231c3c7ce2c9cbb8b436de10e50d1ac7ac09e091d9c0cd6fe88d93db2 dd94a290-a431-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245424 Biotehniška fakulteta 0 0 0