20.500.12556/RUL-186280 Inženirski pristopi za modulacijo mikrobnih biofilmov v biotehnološke namene Engineering approaches for modulating microbial biofilms for biotechnological purposes Biofilmi predstavljajo organizirane mikrobne skupnosti, obdane z zunajceličnim matriksom, ki mikroorganizmom omogoča večjo odpornost proti stresnim dejavnikom in prispeva k večji sposobnosti preživetja. Dolgo časa so bili biofilmi obravnavani predvsem kot nezaželen pojav v medicini, industriji in okoljskih sistemih, medtem ko se v zadnjih letih vse bolj uveljavljajo kot obetavna platforma za različne biotehnološke aplikacije. Namen diplomskega dela je bil predstaviti osnovne značilnosti, sodobne pristope inženiringa biofilmov ter možnosti uporabe biofilmov v biotehnologiji. Pregled literature je pokazal, da je mogoče z usmerjenim spreminjanjem regulatornih mehanizmov, zunajceličnih polimernih snovi (EPS) in okoljskih dejavnikov učinkovito vplivati na nastanek, strukturo in funkcionalnost biofilmov. Pomembno vlogo pri tem imajo tudi genetski inženiring, sintetična biologija ter modifikacija materialov in površin, ki omogočajo razvoj biofilmov z natančno prilagojenimi lastnostmi. Takšni biofilmi imajo velik potencial za uporabo v bioremediaciji, mikrobnih gorivnih celicah, biosenzoriki ter pri razvoju inženirskih živih materialov. Kljub velikemu potencialu za uporabo, ostajajo pomembni izzivi, ki so povezani z nadzorom kompleksnih mikrobnih skupnosti, zagotavljanjem dolgoročne stabilnosti ter varnim prenosom biofilmskih sistemov iz laboratorijskega v industrijsko okolje. Biofilms are organized microbial communities embedded in an extracellular matrix, which provides microorganisms with greater resistance to environmental stressors and improves their survival. For many years, biofilms were viewed primarily as an undesirable phenomenon in medicine, industry, and environmental systems. In recent years, however, they have been recognized as a promising platform for various biotechnological applications. The aim of this thesis was to present the fundamental characteristics of biofilms, modern biofilm engineering approaches, and their potential applications in biotechnology. A review of the literature has shown that targeted modification of regulatory mechanisms, extracellular polymeric substance (EPS) components, and environmental factors can effectively influence biofilm formation, structure, and functionality. Genetic engineering, synthetic biology, and the modification of materials and surfaces play a key role in the development of biofilms with precisely tailored properties. Such engineered biofilms hold significant potential for applications in bioremediation, microbial fuel cells, biosensors, and the development of engineered living materials. Despite their significant potential, major challenges remain, including the control of complex microbial communities, ensuring the long-term stability of biofilm-based systems, and the safe translation of these systems from laboratory research to industrial applications. biofilmi inženiring biofilmov sintetična biologija genetske tarče biotehnološke aplikacije mikrobne gorivne celice biofilms biofilm engineering synthetic biology genetic targets biotechnological applications microbial fuel cells true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-08-30 07:18:19 2026-08-30 07:18:27 2026-09-01 14:13:11 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 286310 289634563 60686.pdf 60686.pdf 1 B3BA085A0321F5335E7E759F4439F55A f91b75c7f3c981b65199f25c47af18c04cf231fea5d364b8c3adf9db4b451045 ce378050-a431-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245423 Biotehniška fakulteta 0 0 0