20.500.12556/RUL-186242 Prilagojenost kvasovke Hanseniaspora valbyensis na fermentativna okolja Adaptability of Hanseniaspora valbyensis yeasts to fermentative environments V magistrskem delu smo raziskali fenotipsko raznolikost 18 sevov kvasovk rodu Hanseniaspora ter povezavo med njihovimi genetskimi lastnostmi, hitrostjo rasti in produkcijo primarnih metabolitov. Seve smo gojili v aerobnih in anaerobnih pogojih ter določili parametre rasti, velikost celic, sposobnost sporulacije in koncentracije primarnih metabolitov - glicerola, ocetne kisline in etanola. Vsi sevi so v aerobnih pogojih rasli hitreje kot v anaerobnih, medtem ko so v anaerobnih pogojih proizvedli višje koncentracije etanola in glicerola ter nižje koncentracije ocetne kisline, kar kaže na ohranjen fermentativni metabolizem. Med ozko sorodnima vrstama Hanseniaspora smithiae in Hanseniaspora valbyensis nismo ugotovili statistično značilnih razlik v hitrosti rasti, vendar je H. valbyensis proizvedla več etanola. Le nekateri sevi vrste H. valbyensis so bili sposobni fermentacije v aerobnih razmerah, kar nakazuje na Crabtree učinek pri teh sevih. Med velikostjo genoma in hitrostjo rasti v aerobnih pogojih smo opazili trend pozitivne korelacije, saj so sevi z večjim genomom praviloma rasli hitreje in proizvedli manj etanola. Analiza genomskih podatkov je pokazala tudi, da prisotnost oz. organizacija mitohondrijskih genov COX1, COX2 in COX3 ni povezana s sposobnostjo tvorbe askospor pri sevih rodu Hanseniaspora. Rezultati kažejo, da je razpoložljivost kisika glavni dejavnik, ki vpliva na hitrost rasti in produkcijo primarnih metabolitov preučevanih sevov, medtem ko genomska raznolikost pomembno prispeva k njihovi fenotipski raznolikosti. In this master's thesis, we investigated the phenotypic diversity of 18 yeast strains belonging to the genus Hanseniaspora and the relationship between their genomic characteristics, growth rate and the production of primary metabolites. The strains were cultivated under aerobic and anaerobic conditions, and growth parameters, cell size, sporulation ability and the concentrations of glycerol, acetic acid and ethanol were determined. All strains exhibited faster growth under aerobic conditions than under anaerobic conditions, while anaerobic cultivation resulted in increased ethanol and glycerol production and decreased acetic acid production, indicating that all strains retained fermentative metabolism. No statistically significant differences in growth rate were observed between the closely related Hanseniaspora smithiae and Hanseniaspora valbyensis. However, H. valbyensis produced higher amounts of ethanol under anaerobic conditions. Increased ethanol production under aerobic conditions was observed in some H. valbyensis strains, suggesting the Crabtree effect. A positive correlation between genome size and maximum specific growth rate was observed. Strains possessing larger genomes grew faster and produced less ethanol under aerobic conditions. Analysis of genomic data also showed that the presence or organization of the mitochondrial genes COX1, COX2 and COX3 was not associated with the ability to form ascospores. The results indicate that oxygen availability is the primary factor affecting growth and primary metabolite production in the studied strains, while genomic diversity contributes to the observed phenotypic diversity. Hanseniaspora valbyensis kvasovke fenotipska raznolikost primarni metaboliti fermentativni metabolizem velikost genoma sporulacija Hanseniaspora valbyensis yeasts phenotypic diversity primary metabolites fermentative metabolism genome size sporulation true false false [L. Staver] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-08-29 07:20:58 2026-08-29 07:21:08 2026-08-31 08:06:30 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.22/.26:582.28.23 286181 289394435 60655.pdf 60655.pdf 1 3875F6DD2DD8DB401D919D884F1F3E8A ce08c9c67c6b399e7f047e4bf704d30d97a05837b7a77ccc465a3a3972569b89 073dd83a-a369-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245380 Biotehniška fakulteta 0 0 0