20.500.12556/RUL-186241 Pregled molekularnih metod za detekcijo glivnih in virusnih povzročiteljev bolezni hmelja (Humulus lupulus) Review of Molecular Methods for Detecting Fungal and Viral Pathogens of Hop (Humulus lupulus) Hmelj (Humulus lupulus) je v Sloveniji pomembna gospodarska rastlina, zlasti v pivovarstvu. Bolezni, ki jih povzročajo glive, virusi ter viroidi, predstavljajo resno oviro pri pridelavi hmelja, zato je pomembna njihova hitra in zanesljiva detekcija. Namen diplomske naloge je pregled in primerjava sodobnih ter molekularnih metod v razvoju za detekcijo najpomembnejših patogenov hmelja (Verticillium spp., Fusarium spp., HLVd, CBCVd, HSVd, HpMV). Klasične vizualne in serološke metode večinoma ne zadostujejo v občutljivosti, še posebej pri začetnih fazah okužb in latentnih okužbah. Zlati standard za detekcijo patogenov pri hmelju tako trenutno predstavljajo metode PCR in njegove nadgradnje (qPCR, RT-qPCR), ki so najpogosteje uporabljene v rutinski diagnostiki in omogočajo specifično in kvantitativno zaznavo patogenov, tudi pri nizkih koncentracijah tarčne nukleinske kisline. Primerjava metod je pokazala, da novejši diagnostični pristopi, kot so metode na osnovi izotermnega pomnoževanja, CRISPR-Cas detekcijski sistemi ter nanomaterialni genosenzorji, kažejo hitro in specifično možnost detekcije na terenu. Boinformacijska orodja na osnovi visokozmogljivega sekvenciranja pa predstavljajo pomembno orodje pri raziskovalni diagnostiki ter identifikaciji novih patogenov. Našteti pristopi imajo velik potencial za uporabo v diagnostiki patogenov pri hmelju, za uporabo pa zahtevajo še nadaljnjo validacijo in standardizacijo. Hop (Humulus lupulus) is an important economic crop in Slovenia, especially in brewing. Diseases caused by fungi, viruses, and viroids represent a serious obstacle for growing hops, which is why their rapid and reliable detection is important. The purpose of this thesis is to review and compare modern and molecular methods under development for the detection of the most important hop pathogens (Verticillium spp., Fusarium spp., HLVd, CBCVd, HSVd, HpMV). Conventional visual and serological methods are generally insufficient in terms of sensitivity, especially in the early stages of infection and latent infections. The gold standard for pathogen detection in hops is currently represented by PCR methods and their upgrades (qPCR, RT-qPCR), which are most commonly used in routine diagnostics and enable specific and quantitative detection of pathogens, even at low concentrations of target nucleic acid. A comparison of methods has shown that newer diagnostic approaches, such as isothermal amplification-based methods, CRISPR-Cas detection systems and nanomaterial genosensors, offer rapid and specific detection possibilities in the field. Bioinformatics tools based on high-throughput sequencing are an important tool in research diagnostics and the identification of new pathogens. These approaches have great potential for use in the diagnosis of pathogens in hops, but require further validation and standardization before their routine application. Humulus lupulus hmelj molekularne metode patogeni detekcija Humulus lupulus hops molecular methods pathogens detection true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-08-29 07:20:13 2026-08-29 07:20:25 2026-08-31 09:37:02 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 286180 289422339 60715.pdf 60715.pdf 1 29EDACA6DD7CA0FE674F8D838E061812 451a551584a616b181ae2c44c61ddda29b399ad0eb7654131cf36445440075f1 ef5d32fb-a368-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245379 Biotehniška fakulteta 0 0 0