20.500.12556/RUL-185991 Uporaba dikloro-N-metil maleimida za fluorescenčno označevanje aminokislin Use of dichloro-N-methyl maleimide for fluorescent labeling of amino acids Za sintezo novih fluorescenčnih standardov označenih α-aminokislin smo uporabili 2,3-diklorido-N-metilmaleimid. Reakcijo vezave 2,3-diklorido-N-metilmaleimida na aminokislinske derivate smo optimizirali. Aminokisline z več kot eno nukleofilno skupino smo ustrezno zaščitili. Označevanju aminokislin je sledilo označevanje oligopeptidov in na koncu proteina. Označene aminokisline in oligopeptide smo spektroskopsko okarakterizirali, pridobljene podatke pa uporabili za funkcionalizacijo in ovrednotenja vezave 2,3-diklorido-N-metilmaleimida na goveji serumski albumin (BSA). We used 2,3-dichloro-N-methylmaleimide to synthesize a library of labeled α-amino acids. When necessary, the amino acids were protected to obtain standards of amino acids labeled at their side chains. Amino acid labeling was followed by the labeling of oligopeptides and, finally, the protein. The labeled amino acids and oligopeptides were spectroscopically characterized, and the resulting data were used to estimate the number of labeled amino acid residues on the surface of bovine serum albumin (BSA). In addition, the stability of 2,3-dichloro-N-methylmaleimide in aqueous buffer systems was evaluated 2 3-diklorido-N-metilmaleimid biokonjugacija fluorescenčno označevanje α-aminokisline BSA 2 3-diklorido-N-metilmaleimid biokonjugacija fluorescenčno označevanje α-aminokisline BSA true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-08-25 10:55:06 2026-08-25 10:55:11 2026-08-26 04:13:06 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 25788 Ostojic_Ivana_-_Uporaba_dikloro-N-metil_maleimida_za_fluorescencno_oznacevanje_aminokislin.pdf Ostojic_Ivana_-_Uporaba_dikloro-N-metil_maleimida_za_fluorescencno_oznacevanje_aminokislin.pdf 1 FEDE5F589DA9A0A4A6C6BB1FB9F2E551 ff793c38759607e2980e13261683b9bb02d8fc10d970fcbf3a211881eb0fc7e2 4780472d-a062-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=245028 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0