20.500.12556/RUL-185888 Izzivi prenosa laboratorijske razgradnje plastike z glivami na industrijski nivo Challenges in the scale-up of fungal biodegradation of plastics Kopičenje plastičnih odpadkov predstavlja enega največjih okoljskih problemov današnjega časa. Zaradi velike obstojnosti sintetičnih polimerov in omejitev obstoječih metod ravnanja z odpadki se vse več pozornosti namenja iskanju alternativnih, okolju prijaznejših načinov razgradnje plastike. Med njimi imajo pomemben potencial glive, ki s pomočjo različnih ekstracelularnih encimov omogočajo razgradnjo določenih vrst plastičnih materialov. Diplomsko delo obravnava mehanizme glivne razgradnje plastike, vlogo glivnih encimov ter sposobnost razgradnje najpogostejših tipov plastike. Poseben poudarek je namenjen izzivom prenosa laboratorijskih raziskav na industrijski nivo. Predstavljeni so biološki, procesni in substratni dejavniki, ki omejujejo učinkovitost procesa v večjem merilu, med drugim počasna hitrost razgradnje, heterogenost plastičnih odpadkov, zahteve glede sterilnosti, omejitve pri prenosu kisika in ekonomski dejavniki. Obravnavane so tudi možnosti za izboljšanje procesa, kot so uporaba učinkovitejših glivnih sevov, mikrobnih združb, gensko in encimsko inženirstvo ter razvoj naprednejših bioreaktorskih sistemov. Ugotovitve kažejo, da glivna razgradnja plastike zaenkrat še ni konkurenčna uveljavljenim načinom ravnanja z odpadki, vendar bi lahko nadaljnji razvoj tehnologije v prihodnosti povečal njeno uporabnost. Plastic waste accumulation represents one of the major environmental challenges of modern society. Due to the high persistence of synthetic polymers and the limitations of current waste management methods, increasing attention is being directed towards the development of alternative and environmentally friendly approaches for plastic degradation. Among these, fungi have attracted considerable interest because they are capable of degrading certain types of plastic materials through the action of various extracellular enzymes. This thesis discusses the mechanisms of fungal plastic biodegradation, the role of fungal enzymes, and the biodegradation potential of the most common plastic types. Particular emphasis is placed on the challenges associated with scaling up laboratory research to industrial applications. Biological, process-related, and substrate-related factors that limit the efficiency of fungal biodegradation at a larger scale are presented, including slow degradation rates, the heterogeneity of plastic waste, sterility requirements, oxygen transfer limitations, and economic constraints. Potential strategies for process improvement are also discussed, such as the use of more efficient fungal strains, microbial consortia, genetic and enzyme engineering, and the development of advanced bioreactor systems. The findings indicate that fungal plastic biodegradation is currently not competitive with established waste management methods; however, further technological advances may significantly increase its applicability in the future. plastika plastični odpadki glive biorazgradnja mikodegradacija encimi prenos na industrijsko merilo bioreaktorji plastic pollution plastic waste fungi biodegradation mycodegradation enzymes scale-up bioreactors true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-08-22 07:16:29 2026-08-22 07:16:37 2026-08-25 11:46:18 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 285420 288790787 60504.pdf 60504.pdf 1 42E9DE05DE1A7821A51B872FB1A64CAD 80a3744514b0de3f18412121208bbb4e12903a6afea7f5e16d49df1c9b881586 416c0ee2-9de8-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=244895 Biotehniška fakulteta 0 0 0