20.500.12556/RUL-185831 Karakterizacija in izolacija bakteriofagnih receptorjev Characterization and isolation of bacteriophage receptors Bakteriofagni receptorji so ključni dejavnik pri prepoznavanju gostitelja in začetku okužbe bakterijske celice, zato njihovo poznavanje pomembno prispeva k razumevanju interakcij med bakteriofagi in bakterijami ter razvoju novih biotehnoloških pristopov. Namen diplomskega dela je predstaviti zgradbo in funkcijo bakteriofagnih receptorjev, mehanizme njihovega prepoznavanja ter pregledati metode njihove karakterizacije in izolacije. Diplomsko delo temelji na pregledu znanstvene literature s področja bakteriofagov, njihovih receptorjev in receptor-vezavnih proteinov. Obravnavani so polisaharidni in proteinski receptorji bakterijskih celic ter njihova vloga pri specifičnosti gostitelja. Predstavljene so genetske metode identifikacije receptorjev, kot so selekcija fagno odpornih mutantov, transpozonsko sekvenciranje in CRISPRi, funkcionalni adsorpcijski testi, biokemijske metode izolacije ter strukturne metode, med katerimi izstopa krioelektronska mikroskopija. Pregled literature kaže, da sodobni pristopi omogočajo natančno identifikacijo bakteriofagnih receptorjev in razumevanje molekularnih interakcij med receptorji ter receptor-vezavnimi proteini. Pridobljeno znanje zagotavlja razvoj učinkovitejše fagne terapije, načrtovanje fagnih koktajlov, razvoj hitrih diagnostičnih metod in novih protimikrobnih strategij. Nadaljnji napredek bo temeljil na povezovanju visokozmogljivih presejalnih metod, strukturne biologije in bioinformatike, kar bo prispevalo k učinkovitejši uporabi bakteriofagov v medicini, veterini in biotehnologiji. Bacteriophage receptors play a crucial role in host recognition and the initiation of bacterial infection, making their characterization essential for understanding bacteriophage–host interactions and advancing novel biotechnological applications. The aim of this bachelor's thesis was to review the structure and function of bacteriophage receptors, the mechanisms underlying receptor recognition, and current methods for their characterization and isolation. The thesis is based on a comprehensive review of scientific literature addressing bacteriophages, bacterial receptors, and receptor-binding proteins. Both polysaccharide and protein receptors and their role in determining host specificity are discussed. The review describes genetic approaches for receptor identification, including the selection of phage-resistant mutants, transposon insertion sequencing, and CRISPR interference, as well as functional adsorption assays, biochemical isolation techniques, and structural methods, particularly cryo-electron microscopy. The reviewed literature demonstrates that modern methodologies enable accurate identification of bacteriophage receptors and provide detailed insight into the molecular interactions between receptors and receptor-binding proteins. This knowledge supports the development of more effective phage therapy, rational design of phage cocktails, rapid diagnostic tools, and novel antimicrobial strategies. Future advances are expected to rely on the integration of high-throughput screening approaches, structural biology, and bioinformatics, further expanding the application of bacteriophages in medicine, veterinary science, and biotechnology. bakteriofagi receptor-vezavni proteini bakterijski receptorji interakcije fag–gostitelj identifikacija receptorjev adsorpcija bakteriofagov bacteriophages receptor-binding proteins bacterial receptors phage–host interactions receptor identification bacteriophage adsorption true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-08-21 08:15:12 2026-08-21 08:15:26 2026-08-22 04:19:29 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 285315 288433667 60320.pdf 60320.pdf 1 75F10BE306976458C80DDDF9987027C2 98be11dce55c853a1560fb45dd229002ed1c3fa1d8d310650b5ea60f021db741 5009e4e9-9d27-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=244820 Biotehniška fakulteta 0 0 0