20.500.12556/RUL-185794 Določanje hidrofobnosti metakrilatnih kromatografskih nosilcev Determination of hydrophobicity of methacrylate chromatographic supports V diplomskem delu smo proučevali, ali so vodotopni sledilci dovolj hidrofobni, da se zadržijo na metakrilatnem nosilcu, ki je šibko hidrofoben zaradi prostih epoksi skupin. Najprej smo določili poroznost in propustnost kolon, saj ti lastnosti vplivata na kromatografsko učinkovitost. Poroznost in propustnost smo določili s spremljanjem prehoda acetona kot sledilca skozi sistem s kolono in brez nje pri različnih pretokih. Hidrofobnost nosilcev smo poskušali določiti z uporabo različnih sledilcev, in sicer lizocima, fenilalanina, tirozina, triptofana, butanola, heksanola in plazmidne DNK. Meritve smo izvedli pri različnih koncentracijah (NH$_4$)$_2$SO$_4$ in različnih vrednostih pH, pri čemer smo za spremljanje prehoda sledilcev uporabljali UV-Vis spektrofotometer. Pri nobenem od uporabljenih vodotopnih sledilcev ni bilo mogoče zanesljivo dokazati popolnega zadržanja na metakrilatnem nosilcu, ki bi ga lahko pripisali hidrofobnim interakcijam. Hidrofobnost metakrilatnega nosilca tako z uporabljenimi sledilci in eksperimentalnimi pogoji nismo mogli določiti. In my diploma thesis, I studied whether water-soluble tracers are hydrophobic enough to be retained on a methacrylate support, which is weakly hydrophobic due to the presence of free epoxy groups. First, I determined the porosity and permeability of the columns, as these properties affect chromatographic performance. The porosity and permeability were determined by monitoring the passage of acetone, used as a tracer, through the system with and without a column at different flow rates. I attempted to determine the hydrophobicity of the supports using various tracers, namely lysozyme, phenylalanine, tyrosine, tryptophan, butanol, hexanol, and plasmid DNA (pDNA). The measurements were performed at different concentrations of ammonium sulfate ((NH$_4$)$_2$SO$_4$) and different pH values, while the passage of the tracers was monitored using a UV–Vis spectrophotometer. None of the water-soluble tracers used provided reliable evidence of complete retention on the methacrylate support that could be attributed to hydrophobic interactions. Therefore, under the applied experimental conditions and using the selected tracers, the hydrophobicity of the methacrylate support could not be determined. metakrilatni nosilec hidrofobnost kromatografija hidrofobnih interakcij. methacrylate support hydrophobicity hydrophobic interaction chromatography. true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-08-20 11:50:04 2026-08-20 11:50:11 2026-09-03 12:06:55 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 26160 289907203 Popelar_Anzej_-_Dolocanje_hidrofobnosti_metakrilatnih_kromatografskih_nosilcev.pdf Popelar_Anzej_-_Dolocanje_hidrofobnosti_metakrilatnih_kromatografskih_nosilcev.pdf 1 A1E6956BC5E7825EBC76FAED945EC5BA 00a14db1d0e7b5cae9c4c73d2443e133bc6b9a44e0ddea0f267fd465d1a8dbc2 261a5538-9c7c-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=244768 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0