20.500.12556/RUL-185775 Določanje števila veznih mest bakteriofaga T4 s kromatografskim nosilcem Determination of number of binding sites with chromatographic support for bacteriophage T4 Za diplomsko nalogo sem proučevala vezavo bakteriofaga T4 na QA polyHIPE kromatografski nosilec. Namen naloge je bil določiti število veznih mest bakteriofaga T4 na nosilcu, pri čemer sem kot modelni protein uporabila protein BSA. Eksperimentalni del naloge je bil izveden na HPLC sistemu s pomočjo ionsko-izmenjevalne kromatografije z gradientno elucijo. Rezultate sem analizirala po Yamamoto modelu. Rezultati so pokazali večje število veznih mest pri fagu T4 kot pri proteinu, kar je pričakovano, saj ima fag zaradi večje in kompleksnejše površine več potencialnih mest za vezavo na nosilec. For my thesis, I studied the binding of bacteriophage T4 to a QA polyHIPE chromatography support. The aim of the of the study was to determine the number of binding sites of phage T4 on the support, using BSA as a model protein. The experimental part was performed on a HPLC system using gradient elution IEX. The results were analysed with Yamamoto model. The results showed a higher number of binding sites for phage T4 than for the protein, which was expected, as the phage has a larger and more complex surface with more potential binding sites on the support. protein BSA bakteriofag T4 ionsko izmenjevalna kromatografija Yamamoto model protein BSA bacteriophage T4 ion-exchange chromatography Yamamoto model true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-08-20 09:20:06 2026-08-20 09:20:14 2026-09-03 10:18:01 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 25594 289866243 Kante_Ariana_-_Dolocanje_stevila_veznih_mest_bakteriofaga_T4_s_kromatografskim_nosilcem.pdf Kante_Ariana_-_Dolocanje_stevila_veznih_mest_bakteriofaga_T4_s_kromatografskim_nosilcem.pdf 1 E247AE7E30F7BC3FCDC93469508EAF38 e6f9f55c5ac7c90e322913779424aedcf069afba26b6a137b2a33346a1a34932 39b43ab7-9c67-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=244701 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0