20.500.12556/RUL-185540 Plasma extracellular vesicle characteristics as biomarkers of resectability and radicality of surgical resection in pancreatic cancer - a prospective cohort study Due to possible diagnostic misjudgment of tumor resectability, patients with pancreatic ductal adenocarcinoma (PDAC) might be exposed to non-radical resection or unnecessary laparotomy. With small extracellular vesicles (sEV) obtained by liquid biopsy, we aimed to evaluate their potential as biomarkers of tumor resectability, radicality of resection and overall survival (OS). Our prospective study included 83 PDAC patients undergoing surgery with curative intent followed-up longitudinally. sEV were isolated from plasma, and their concentration and size were determined. Fifty patients underwent PDAC resection, and thirty-three had no resection. Preoperatively, patients undergoing resection had higher sEV concentrations than those without resection (p = 0.023). Resection was predicted at the cutoff value of 1.88 × 109/mL for preoperative sEV concentration (p = 0.023) and the cutoff value of 194.8 nm for preoperative mean diameter (p = 0.057). Furthermore, patients with R0 resection demonstrated higher preoperative plasma sEV concentrations than patients with R1/R2 resection (p = 0.014). If sEV concentration was above 1.88 × 109/mL or if the mean diameter was below 194.8 nm, patients had significantly longer OS (p = 0.018 and p = 0.030, respectively). Our proof-of-principle study identified preoperative sEV characteristics as putative biomarkers of feasibility and radicality of PDAC resection that also enable discrimination of patients with worse OS. Liquid biopsy with sEV could aid in PDAC patient stratification and treatment optimization in the future. zunajcelični vezikli duktalni adenokarcinom trebušne slinavke resekcija extracellular vesicles pancreatic ductal adenocarcinoma resection zunajcelični vezikli duktalni adenokarcinom trebušne slinavke resekcija true false true Angleški jezik Slovenski jezik Članek v reviji 2026-08-08 07:44:20 2026-08-10 08:59:00 2026-08-11 04:01:53 0000-00-00 00:00:00 2023 0 0 15 str. iss. 3 Vol. 15 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2072-6694 10.3390/cancers15030605 140408067 RAZ_Badovinac_David_2023.pdf RAZ_Badovinac_David_2023.pdf 1 22011E92B39D3B5789FBDE097E362357 e2198270845066c0c16ecb002cf3fbfe55b08fe258575ca739961aad817773de 7e991b5a-9488-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=244195 https://www.mdpi.com/2072-6694/15/3/605 1 def48fbe-92eb-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=244161 Medicinska fakulteta 0 0 0